Values CatLog read from this paper
Mutagenesis evidence that the partial reactions of firefly bioluminescence are catalyzed by different conformations of the luciferase C-terminal domain
PMID 15683224doi:10.1021/bi047903fOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (43 values) from this paper: luciferase (Luc) from Photinus pyralis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| luciferase (Luc) | Photinus pyralis | D-firefly luciferin | 0.167 | 0.015 | 11 calc. | pH 7.8 | Open record: luciferase (Luc), D-firefly luciferin |
| luciferase (Luc) | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 0.23 | 0.0047 | 48.9 | pH 7.8 | Open record: luciferase (Luc), D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc) | Photinus pyralis | dehydroluciferin | 12 | — | — | pH 7.8 | Open record: luciferase (Luc), dehydroluciferin |
| luciferase (Luc) | Photinus pyralis | Mg-ATP | — | 0.16 | — | pH 7.8 | Open record: luciferase (Luc), Mg-ATP |
| luciferase (Luc)variant G446I | Photinus pyralis | D-firefly luciferin | 0.02 | 0.006 | 3.3 calc. | pH 7.8 | Open record: luciferase (Luc) variant G446I, D-firefly luciferin |
| luciferase (Luc)variant G446I | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 0.021 | 0.0015 | 9.5 | pH 7.8 | Open record: luciferase (Luc) variant G446I, D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc)variant G446I | Photinus pyralis | Mg-ATP | — | 0.073 | — | pH 7.8 | Open record: luciferase (Luc) variant G446I, Mg-ATP |
| luciferase (Luc)variant K443A | Photinus pyralis | D-firefly luciferin | 6.29 × 10⁻⁵ | 0.0023 | 0.027 calc. | pH 7.8 | Open record: luciferase (Luc) variant K443A, D-firefly luciferin |
| luciferase (Luc)variant K443A | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 1.01 × 10⁻⁴ | 3.8 × 10⁻⁴ | 0.27 | pH 7.8 | Open record: luciferase (Luc) variant K443A, D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc)variant K443A | Photinus pyralis | Mg-ATP | — | 0.0486 | — | pH 7.8 | Open record: luciferase (Luc) variant K443A, Mg-ATP |
| luciferase (Luc)variant K443A/K529A | Photinus pyralis | D-firefly luciferin | 1.57 × 10⁻⁷ | 0.067 | 2.3 × 10⁻⁶ calc. | pH 7.8 | Open record: luciferase (Luc) variant K443A/K529A, D-firefly luciferin |
| luciferase (Luc)variant K443A/K529A | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 3.11 × 10⁻⁵ | 5.5 × 10⁻⁴ | 0.057 | pH 7.8 | Open record: luciferase (Luc) variant K443A/K529A, D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc)variant K443A/K529A | Photinus pyralis | Mg-ATP | — | 0.56 | — | pH 7.8 | Open record: luciferase (Luc) variant K443A/K529A, Mg-ATP |
| luciferase (Luc)variant K445Q | Photinus pyralis | D-firefly luciferin | 0.24 | 0.0089 | 27 calc. | pH 7.8 | Open record: luciferase (Luc) variant K445Q, D-firefly luciferin |
| luciferase (Luc)variant K445Q | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 0.32 | 0.0039 | 82 | pH 7.8 | Open record: luciferase (Luc) variant K445Q, D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc)variant K445Q | Photinus pyralis | Mg-ATP | — | 0.069 | — | pH 7.8 | Open record: luciferase (Luc) variant K445Q, Mg-ATP |
| luciferase (Luc)variant K529A | Photinus pyralis | D-firefly luciferin | 2.5 × 10⁻⁴ | 0.23 | 0.0011 calc. | pH 7.8 | Open record: luciferase (Luc) variant K529A, D-firefly luciferin |
| luciferase (Luc)variant K529A | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 0.175 | 0.0126 | 13.9 | pH 7.8 | Open record: luciferase (Luc) variant K529A, D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc)variant K529A | Photinus pyralis | Mg-ATP | — | 1.2 | — | pH 7.8 | Open record: luciferase (Luc) variant K529A, Mg-ATP |
| luciferase (Luc)variant Q448A | Photinus pyralis | D-firefly luciferin | 0.0875 | 0.006 | 15 calc. | pH 7.8 | Open record: luciferase (Luc) variant Q448A, D-firefly luciferin |
| luciferase (Luc)variant Q448A | Photinus pyralis | D-luciferyl-O-adenosine monophosphate | 0.095 | 0.0022 | 43.2 | pH 7.8 | Open record: luciferase (Luc) variant Q448A, D-luciferyl-O-adenosine monophosphate |
| luciferase (Luc)variant Q448A | Photinus pyralis | Mg-ATP | — | 0.341 | — | pH 7.8 | Open record: luciferase (Luc) variant Q448A, Mg-ATP |