CatLogenzyme kinetics catalog

EC 1.14.11.45: L-isoleucine 4-hydroxylase

OxidoreductasesOpen in Browse

CatLog read 8 records for EC 1.14.11.45 from 1 paper, for 1 organism. Names in these papers: L-isoleucine dioxygenase (Ido).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 11 records for EC 1.14.11.45 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.11.45 in Browse.

Papers

PaperOrganismsRecords
Directed evolution and site-specific mutagenesis of L-isoleucine dioxygenase derived from Bacillus weihenstephanensisBacillus weihenstephanensis8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
L-isoleucine dioxygenase (Ido)Bacillus weihenstephanensis2-oxoglutaric acid (α-ketoglutaric acid)0.57652.760.208830 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)Bacillus weihenstephanensisL-isoleucine0.48052.380.202530 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)variant N126HBacillus weihenstephanensis2-oxoglutaric acid (α-ketoglutaric acid)0.31830.50.636730 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)variant N126HBacillus weihenstephanensisL-isoleucine0.28870.520.559730 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)variant N126H/T130KBacillus weihenstephanensis2-oxoglutaric acid (α-ketoglutaric acid)0.52650.850.621730 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)variant N126H/T130KBacillus weihenstephanensisL-isoleucine0.73581.510.488530 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)variant T130KBacillus weihenstephanensis2-oxoglutaric acid (α-ketoglutaric acid)0.30882.010.153730 °CPMID 29869760Open
L-isoleucine dioxygenase (Ido)variant T130KBacillus weihenstephanensisL-isoleucine0.50183.340.150230 °CPMID 29869760Open