Values CatLog read from this paper
Directed evolution and site-specific mutagenesis of L-isoleucine dioxygenase derived from Bacillus weihenstephanensis
PMID 29869760doi:10.1007/s10529-018-2566-8Open in Browse
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CatLog read 8 records (24 values) from this paper: L-isoleucine dioxygenase (Ido) from Bacillus weihenstephanensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-isoleucine dioxygenase (Ido) | Bacillus weihenstephanensis | 2-oxoglutaric acid (α-ketoglutaric acid) | 0.5765 | 2.76 | 0.2088 | 30 °C | Open record: L-isoleucine dioxygenase (Ido), 2-oxoglutaric acid (α-ketoglutaric acid) |
| L-isoleucine dioxygenase (Ido) | Bacillus weihenstephanensis | L-isoleucine | 0.4805 | 2.38 | 0.2025 | 30 °C | Open record: L-isoleucine dioxygenase (Ido), L-isoleucine |
| L-isoleucine dioxygenase (Ido)variant N126H | Bacillus weihenstephanensis | 2-oxoglutaric acid (α-ketoglutaric acid) | 0.3183 | 0.5 | 0.6367 | 30 °C | Open record: L-isoleucine dioxygenase (Ido) variant N126H, 2-oxoglutaric acid (α-ketoglutaric acid) |
| L-isoleucine dioxygenase (Ido)variant N126H | Bacillus weihenstephanensis | L-isoleucine | 0.2887 | 0.52 | 0.5597 | 30 °C | Open record: L-isoleucine dioxygenase (Ido) variant N126H, L-isoleucine |
| L-isoleucine dioxygenase (Ido)variant N126H/T130K | Bacillus weihenstephanensis | 2-oxoglutaric acid (α-ketoglutaric acid) | 0.5265 | 0.85 | 0.6217 | 30 °C | Open record: L-isoleucine dioxygenase (Ido) variant N126H/T130K, 2-oxoglutaric acid (α-ketoglutaric acid) |
| L-isoleucine dioxygenase (Ido)variant N126H/T130K | Bacillus weihenstephanensis | L-isoleucine | 0.7358 | 1.51 | 0.4885 | 30 °C | Open record: L-isoleucine dioxygenase (Ido) variant N126H/T130K, L-isoleucine |
| L-isoleucine dioxygenase (Ido)variant T130K | Bacillus weihenstephanensis | 2-oxoglutaric acid (α-ketoglutaric acid) | 0.3088 | 2.01 | 0.1537 | 30 °C | Open record: L-isoleucine dioxygenase (Ido) variant T130K, 2-oxoglutaric acid (α-ketoglutaric acid) |
| L-isoleucine dioxygenase (Ido)variant T130K | Bacillus weihenstephanensis | L-isoleucine | 0.5018 | 3.34 | 0.1502 | 30 °C | Open record: L-isoleucine dioxygenase (Ido) variant T130K, L-isoleucine |