CatLogenzyme kinetics catalog

EC 1.14.12.17: nitric oxide dioxygenase

OxidoreductasesOpen in Browse

CatLog read 24 records for EC 1.14.12.17 from 2 papers, for 3 organisms. Names in these papers: flavohemoglobin (nitric-oxide dioxygenase, NOD), flavohemoglobin (NO dioxygenase, flavoHb).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 14 records for EC 1.14.12.17 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.12.17 in Browse.

Papers

PaperOrganismsRecords
Steady-state and transient kinetics of Escherichia coli nitric-oxide dioxygenase (flavohemoglobin). The B10 tyrosine hydroxyl is essential for dioxygen binding and catalysisEscherichia coli13
Nitric-oxide dioxygenase activity and function of flavohemoglobins. sensitivity to nitric oxide and carbon monoxide inhibitionAlcaligenes eutrophus, Escherichia coli and 1 more11

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygensaturating 1 µM NO; 100 µM NADH (Fig. 3A line 1)—0.1—pH 7.8, 37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygenlow 0.1 µM NO; 100 µM NADH (Fig. 3A); cosubstrate NO; NADH—0.035—pH 7.8, 37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygensaturating 1 µM NO; 600 µM NADPH (Fig. 3A line 2)—0.1—pH 7.8, 37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygenlow 0.1 µM NO—0.013—pH 7.8, 20 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygenNO varied at 200; 50 and 25 µM O2; 100 µM NADH—0.08—pH 7.8, 37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygenhigh 1 µM NO—0.0273.5 × 10³pH 7.8, 20 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colidioxygen—0.06—37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia coliNADH5000.00481.0 × 10⁵ calc.pH 7.8, 37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia coliNADH830.00322.6 × 10⁴ calc.pH 7.8, 20 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia coliNADPH—0.18—pH 7.8, 20 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colinitric oxide6702.8 × 10⁻⁴2.4 × 10⁶pH 7.8, 37 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colinitric oxide941.1 × 10⁻⁴8.5 × 10⁵pH 7.8, 20 °CPMID 10777548Open
flavohemoglobin (nitric-oxide dioxygenase, NOD)Escherichia colinitric oxide—4 × 10⁻⁴—37 °CPMID 10777548Open
flavohemoglobin (NO dioxygenase, flavoHb)Alcaligenes eutrophusdioxygen—0.08—pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Alcaligenes eutrophusNADH2900.074.1 × 10³ calc.pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Alcaligenes eutrophusnitric oxide—1 × 10⁻⁴—pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Escherichia colidioxygen—0.09—pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Escherichia coliNADH3650.0031.2 × 10⁵ calc.pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Escherichia coliNADPH2000.141.4 × 10³ calc.pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Escherichia colinitric oxide—2.5 × 10⁻⁴—pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Saccharomyces cerevisiaedioxygen—0.06—pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Saccharomyces cerevisiaeNADH1120.0284.0 × 10³ calc.pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Saccharomyces cerevisiaeNADPH930.39240 calc.pH 7.8, 37 °CPMID 10922365Open
flavohemoglobin (NO dioxygenase, flavoHb)Saccharomyces cerevisiaenitric oxide—1.3 × 10⁻⁴—pH 7.8, 37 °CPMID 10922365Open