Values CatLog read from this paper
Steady-state and transient kinetics of Escherichia coli nitric-oxide dioxygenase (flavohemoglobin). The B10 tyrosine hydroxyl is essential for dioxygen binding and catalysis
PMID 10777548doi:10.1074/jbc.275.17.12581Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (20 values) from this paper: flavohemoglobin (nitric-oxide dioxygenase, NOD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygensaturating 1 µM NO; 100 µM NADH (Fig. 3A line 1) | — | 0.1 | — | pH 7.8, 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygenlow 0.1 µM NO; 100 µM NADH (Fig. 3A); cosubstrate NO; NADH | — | 0.035 | — | pH 7.8, 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygensaturating 1 µM NO; 600 µM NADPH (Fig. 3A line 2) | — | 0.1 | — | pH 7.8, 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygenlow 0.1 µM NO | — | 0.013 | — | pH 7.8, 20 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygenNO varied at 200; 50 and 25 µM O2; 100 µM NADH | — | 0.08 | — | pH 7.8, 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygenhigh 1 µM NO | — | 0.027 | 3.5 × 10³ | pH 7.8, 20 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | dioxygen | — | 0.06 | — | 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), dioxygen |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | NADH | 500 | 0.0048 | 1.0 × 10⁵ calc. | pH 7.8, 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), NADH |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | NADH | 83 | 0.0032 | 2.6 × 10⁴ calc. | pH 7.8, 20 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), NADH |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | NADPH | — | 0.18 | — | pH 7.8, 20 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), NADPH |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | nitric oxide | 670 | 2.8 × 10⁻⁴ | 2.4 × 10⁶ | pH 7.8, 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), nitric oxide |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | nitric oxide | 94 | 1.1 × 10⁻⁴ | 8.5 × 10⁵ | pH 7.8, 20 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), nitric oxide |
| flavohemoglobin (nitric-oxide dioxygenase, NOD) | Escherichia coli | nitric oxide | — | 4 × 10⁻⁴ | — | 37 °C | Open record: flavohemoglobin (nitric-oxide dioxygenase, NOD), nitric oxide |