EC 1.14.13.196: L-ornithine N5-monooxygenase [NAD(P)H]
OxidoreductasesOpen in Browse
CatLog read 28 records for EC 1.14.13.196 from 3 papers, for 1 organism. Names in these papers: SidA ornithine N5-monooxygenase, Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), SidA (ornithine N5-monooxygenase).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 1 record for EC 1.14.13.196 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.13.196 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Contribution to catalysis of ornithine binding residues in ornithine N5-monooxygenase | Aspergillus fumigatus | 12 |
| Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor | Aspergillus fumigatus | 8 |
| Role of Ser-257 in the sliding mechanism of NADP(H) in the reaction catalyzed by the Aspergillus fumigatus flavin-dependent ornithine N5-monooxygenase SidA | Aspergillus fumigatus | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | L-ornithineNADH fixed at 1 mM; cosubstrate NADH; Table 2 | 0.5 | 1.7 | 0.3 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | L-ornithineNADPH fixed at 1 mM; cosubstrate NADPH; apparent kcat | 0.48 | 1.7 | 0.28 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | L-ornithineoxygen consumption assay (Hansatech Oxygraph); Table 3 | 0.68 | 0.5 | 1.33 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | L-ornithineoxygen consumption assay (Hansatech Oxygraph); Table 3 | 0.87 | 2 | 0.43 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | NADHL-ornithine saturating at 15 mM | 1.18 | 0.9 | 1.32 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADH |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | NADHoxygen consumption assay (Hansatech Oxygraph) | 0.73 | 0.21 | 3.42 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADH |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | NADPHL-ornithine saturating at 15 mM | 1.25 | 0.94 | 1.32 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADPH |
| Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) | Aspergillus fumigatus | NADPHoxygen consumption assay (Hansatech Oxygraph) | 0.63 | 0.03 | 21.05 | pH 7.5, 25 °C | PMID 20614882 | Open record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADPH |
| SidA (ornithine N5-monooxygenase) | Aspergillus fumigatus | L-ornithineNADPH 1.0 mM; cosubstrate NADPH (1.0 mM) | 0.45 | 0.8 | 0.54 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase), L-ornithine |
| SidA (ornithine N5-monooxygenase) | Aspergillus fumigatus | L-ornithineAPADPH 1.0 mM; cosubstrate APADPH (1.0 mM) | 0.03 | 0.3 | 0.14 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase), L-ornithine |
| SidA (ornithine N5-monooxygenase) | Aspergillus fumigatus | NADPH | 0.6 | 0.017 | 14 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase), NADPH |
| SidA (ornithine N5-monooxygenase) | Aspergillus fumigatus | reduced 3-acetylpyridine-adenine dinucleotide phosphate | 0.25 | 0.14 | 1.8 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase), reduced 3-acetylpyridine-adenine dinucleotide phosphate |
| SidA (ornithine N5-monooxygenase)variant S257A | Aspergillus fumigatus | L-ornithineNADPH 1.0 mM; cosubstrate NADPH (1.0 mM) | 0.12 | 0.34 | 0.34 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase) variant S257A, L-ornithine |
| SidA (ornithine N5-monooxygenase)variant S257A | Aspergillus fumigatus | L-ornithineAPADPH 1.0 mM; cosubstrate APADPH (1.0 mM) | 0.03 | 0.21 | 0.09 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase) variant S257A, L-ornithine |
| SidA (ornithine N5-monooxygenase)variant S257A | Aspergillus fumigatus | NADPH | 0.85 | 0.014 | 40 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase) variant S257A, NADPH |
| SidA (ornithine N5-monooxygenase)variant S257A | Aspergillus fumigatus | reduced 3-acetylpyridine-adenine dinucleotide phosphate | 0.27 | 0.067 | 4 | pH 7.5, 25 °C | PMID 24072704 | Open record: SidA (ornithine N5-monooxygenase) variant S257A, reduced 3-acetylpyridine-adenine dinucleotide phosphate |
| SidA ornithine N5-monooxygenase | Aspergillus fumigatus | L-ornithineoxygen consumption assay (Hansatech Oxygraph) | 0.59 | 1.1 | 0.54 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase, L-ornithine |
| SidA ornithine N5-monooxygenase | Aspergillus fumigatus | L-ornithineOrn hydroxylation assay (Csaky iodine oxidation) | 0.62 | 1 | 0.6 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase, L-ornithine |
| SidA ornithine N5-monooxygenase | Aspergillus fumigatus | NADPH | — | 0.007 | 84 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase, NADPH |
| SidA ornithine N5-monooxygenasevariant N293A | Aspergillus fumigatus | L-ornithineoxygen consumption assay (Hansatech Oxygraph) | 0.88 | 18 | 0.05 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant N293A, L-ornithine |
| SidA ornithine N5-monooxygenasevariant N293A | Aspergillus fumigatus | L-ornithineOrn hydroxylation assay (Csaky iodine oxidation) | 0.53 | 16 | 0.033 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant N293A, L-ornithine |
| SidA ornithine N5-monooxygenasevariant N293A | Aspergillus fumigatus | NADPH | — | 0.06 | 15 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant N293A, NADPH |
| SidA ornithine N5-monooxygenasevariant N323A | Aspergillus fumigatus | L-ornithineoxygen consumption assay (Hansatech Oxygraph) | 1.06 | 15 | 0.071 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant N323A, L-ornithine |
| SidA ornithine N5-monooxygenasevariant N323A | Aspergillus fumigatus | L-ornithineOrn hydroxylation assay (Csaky iodine oxidation) | 0.5 | 12 | 0.042 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant N323A, L-ornithine |
| SidA ornithine N5-monooxygenasevariant N323A | Aspergillus fumigatus | NADPH | — | 0.01 | 110 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant N323A, NADPH |
| SidA ornithine N5-monooxygenasevariant S469A | Aspergillus fumigatus | L-ornithineoxygen consumption assay (Hansatech Oxygraph) | 0.15 | 21 | 0.0071 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant S469A, L-ornithine |
| SidA ornithine N5-monooxygenasevariant S469A | Aspergillus fumigatus | L-ornithineOrn hydroxylation assay (Csaky iodine oxidation) | 0.107 | 18 | 0.006 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant S469A, L-ornithine |
| SidA ornithine N5-monooxygenasevariant S469A | Aspergillus fumigatus | NADPH | — | 0.025 | 6 | pH 7.5, 25 °C | PMID 26375201 | Open record: SidA ornithine N5-monooxygenase variant S469A, NADPH |