Values CatLog read from this paper

Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor

PMID 20614882doi:10.1021/bi100291nOpen in Browse

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CatLog read 8 records (24 values) from this paper: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase) from Aspergillus fumigatus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.14.13.196

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusL-ornithineNADH fixed at 1 mM; cosubstrate NADH; Table 20.51.70.3pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusL-ornithineNADPH fixed at 1 mM; cosubstrate NADPH; apparent kcat0.481.70.28pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusL-ornithineoxygen consumption assay (Hansatech Oxygraph); Table 30.680.51.33pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusL-ornithineoxygen consumption assay (Hansatech Oxygraph); Table 30.8720.43pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), L-ornithine
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusNADHL-ornithine saturating at 15 mM1.180.91.32pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADH
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusNADHoxygen consumption assay (Hansatech Oxygraph)0.730.213.42pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADH
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusNADPHL-ornithine saturating at 15 mM1.250.941.32pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADPH
Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase)Aspergillus fumigatusNADPHoxygen consumption assay (Hansatech Oxygraph)0.630.0321.05pH 7.5, 25 °COpen record: Af SidA (L-ornithine N5-hydroxylase, flavin-dependent monooxygenase), NADPH