CatLogenzyme kinetics catalog

EC 1.14.13.242: 3-hydroxy-2-methylpyridine-5-carboxylate monooxygenase

OxidoreductasesOpen in Browse

CatLog read 9 records for EC 1.14.13.242 from 1 paper, for 1 organism. Names in these papers: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 18 records for EC 1.14.13.242 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.13.242 in Browse.

Papers

PaperOrganismsRecords
Role of the Tyr270 residue in 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium lotiMesorhizobium loti9

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)Mesorhizobium loti5-hydroxynicotinic acid1.3 × 10³0.062.2 × 10⁴pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)Mesorhizobium lotiNADHring-opening activity; 0.2 mM 5HN; cosubstrate 5HN—0.1—pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)Mesorhizobium lotiNADHbasal NADH oxidation uncoupled from ring opening0.191.20.16pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270AMesorhizobium loti5-hydroxynicotinic acid0.050.0451.1pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270AMesorhizobium lotiNADHring-opening activity; 0.2 mM 5HN; cosubstrate 5HN—0.47—pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270AMesorhizobium lotiNADHbasal NADH oxidation uncoupled from ring opening0.840.531.6pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270FMesorhizobium loti5-hydroxynicotinic acid190.2191pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270FMesorhizobium lotiNADHring-opening activity; 7 mM 5HN; cosubstrate 5HN—0.12—pH 8, 25 °CPMID 27568368Open
2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270FMesorhizobium lotiNADHbasal NADH oxidation uncoupled from ring opening7.40.3124pH 8, 25 °CPMID 27568368Open