Values CatLog read from this paper
Role of the Tyr270 residue in 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti
PMID 27568368doi:10.1016/j.jbiosc.2016.07.022Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (21 values) from this paper: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) from Mesorhizobium loti.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) | Mesorhizobium loti | 5-hydroxynicotinic acid | 1.3 × 10³ | 0.06 | 2.2 × 10⁴ | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO), 5-hydroxynicotinic acid |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) | Mesorhizobium loti | NADHring-opening activity; 0.2 mM 5HN; cosubstrate 5HN | — | 0.1 | — | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO), NADH |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) | Mesorhizobium loti | NADHbasal NADH oxidation uncoupled from ring opening | 0.19 | 1.2 | 0.16 | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO), NADH |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270A | Mesorhizobium loti | 5-hydroxynicotinic acid | 0.05 | 0.045 | 1.1 | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) variant Y270A, 5-hydroxynicotinic acid |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270A | Mesorhizobium loti | NADHring-opening activity; 0.2 mM 5HN; cosubstrate 5HN | — | 0.47 | — | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) variant Y270A, NADH |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270A | Mesorhizobium loti | NADHbasal NADH oxidation uncoupled from ring opening | 0.84 | 0.53 | 1.6 | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) variant Y270A, NADH |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270F | Mesorhizobium loti | 5-hydroxynicotinic acid | 19 | 0.21 | 91 | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) variant Y270F, 5-hydroxynicotinic acid |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270F | Mesorhizobium loti | NADHring-opening activity; 7 mM 5HN; cosubstrate 5HN | — | 0.12 | — | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) variant Y270F, NADH |
| 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO)variant Y270F | Mesorhizobium loti | NADHbasal NADH oxidation uncoupled from ring opening | 7.4 | 0.31 | 24 | pH 8, 25 °C | Open record: 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO) variant Y270F, NADH |