EC 1.14.99.66: [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase
OxidoreductasesOpen in Browse
CatLog read 18 records for EC 1.14.99.66 from 3 papers, for 2 organisms. Names in these papers: LSD2, histone demethylase LSD1 (lysine-specific demethylase 1), lysine-specific demethylase 1A (KDM1A/LSD1).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 2 records for EC 1.14.99.66 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.99.66 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| A novel mammalian flavin-dependent histone demethylase | Mus musculus | 9 |
| Human histone demethylase LSD1 reads the histone code | Homo sapiens | 6 |
| Lysine-Specific Demethylase 1A (KDM1A/LSD1): Product Recognition and Kinetic Analysis of Full-Length Histones | Homo sapiens | 3 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| histone demethylase LSD1 (lysine-specific demethylase 1) | Homo sapiens | histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) dimethylated on Lys450 mM Hepes/NaOH; no added salt (ionic strength 22 mM) | 0.135 | 0.0042 | 32 | pH 7.5, 25 °C | PMID 16223729 | Open record: histone demethylase LSD1 (lysine-specific demethylase 1), histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) dimethylated on Lys4 |
| histone demethylase LSD1 (lysine-specific demethylase 1) | Homo sapiens | histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) dimethylated on Lys450 mM Hepes/NaOH plus 80 mM NaCl | 0.118 | 0.0128 | 9.2 | pH 7.5, 25 °C | PMID 16223729 | Open record: histone demethylase LSD1 (lysine-specific demethylase 1), histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) dimethylated on Lys4 |
| histone demethylase LSD1 (lysine-specific demethylase 1) | Homo sapiens | histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) monomethylated on Lys4 | 0.053 | 0.003 | 18 | pH 7.5, 25 °C | PMID 16223729 | Open record: histone demethylase LSD1 (lysine-specific demethylase 1), histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) monomethylated on Lys4 |
| histone demethylase LSD1 (lysine-specific demethylase 1) | Homo sapiens | histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) monomethylated on Lys4 and acetylated on Lys9 | 0.068 | 0.0175 | 3.8 | pH 7.5, 25 °C | PMID 16223729 | Open record: histone demethylase LSD1 (lysine-specific demethylase 1), histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) monomethylated on Lys4 and acetylated on Lys9 |
| histone demethylase LSD1 (lysine-specific demethylase 1) | Homo sapiens | histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) monomethylated on Lys4 and monomethylated on Lys9 | 0.093 | 0.0039 | 23 | pH 7.5, 25 °C | PMID 16223729 | Open record: histone demethylase LSD1 (lysine-specific demethylase 1), histone H3 N-terminal 21-amino-acid peptide (H3 residues 1-21) monomethylated on Lys4 and monomethylated on Lys9 |
| histone demethylase LSD1 (lysine-specific demethylase 1) | Homo sapiens | histone H3 N-terminal 30-amino-acid peptide (H3 residues 1-30) monomethylated on Lys4 | 0.048 | 0.0034 | 13 | pH 7.5, 25 °C | PMID 16223729 | Open record: histone demethylase LSD1 (lysine-specific demethylase 1), histone H3 N-terminal 30-amino-acid peptide (H3 residues 1-30) monomethylated on Lys4 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21 with Pro16 replaced by Ala, monomethylated on Lys4 | 0.0043 | 0.0125 | 0.35 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21 with Pro16 replaced by Ala, monomethylated on Lys4 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, dimethylated on Lys4apparent kcat; apparent Km | 0.0333 | 0.0113 | 2.95 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, dimethylated on Lys4 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, dimethylated on Lys4 | 0.043 | 0.0062 | 7.0 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, dimethylated on Lys4 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, dimethylated on Lys4 and Lys9 | 0.0222 | 0.0066 | 3.4 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, dimethylated on Lys4 and Lys9 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 | 0.0047 | 0.0092 | 0.51 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 and acetylated on Lys9 | 0.0058 | 0.0705 | 0.083 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 and acetylated on Lys9 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 and Arg17 | 0.005 | 0.0081 | 0.62 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 and Arg17 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 and Lys9 | 0.004 | 0.009 | 0.44 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-21, monomethylated on Lys4 and Lys9 |
| LSD2 | Mus musculus | histone H3 N-terminal peptide residues 1-30, monomethylated on Lys4 | 0.0068 | 0.0051 | 1.3 calc. | pH 8.5, 25 °C | PMID 19407342 | Open record: LSD2, histone H3 N-terminal peptide residues 1-30, monomethylated on Lys4 |
| lysine-specific demethylase 1A (KDM1A/LSD1) | Homo sapiens | histone H3 N-terminal peptide residues 1-21 with dimethylated lysine 4, plus a C-terminal GYG | 0.1 | 0.00446 | 22.5 | pH 7.5, 25 °C | PMID 26673564 | Open record: lysine-specific demethylase 1A (KDM1A/LSD1), histone H3 N-terminal peptide residues 1-21 with dimethylated lysine 4, plus a C-terminal GYG |
| lysine-specific demethylase 1A (KDM1A/LSD1) | Homo sapiens | histone H3 N-terminal peptide residues 1-21 with dimethylated lysine 4, plus a C-terminal GYGcomplex purified by gel filtration | 0.157 | 0.00889 | 17.7 | pH 7.5, 25 °C | PMID 26673564 | Open record: lysine-specific demethylase 1A (KDM1A/LSD1), histone H3 N-terminal peptide residues 1-21 with dimethylated lysine 4, plus a C-terminal GYG |
| lysine-specific demethylase 1A (KDM1A/LSD1) | Homo sapiens | histone H3 N-terminal peptide residues 1-21 with dimethylated lysine 4, plus a C-terminal GYGcomplex purified by gel filtration | 0.159 | 0.00814 | 19.5 | pH 7.5, 25 °C | PMID 26673564 | Open record: lysine-specific demethylase 1A (KDM1A/LSD1), histone H3 N-terminal peptide residues 1-21 with dimethylated lysine 4, plus a C-terminal GYG |