EC 1.2.1.2: formate dehydrogenase

OxidoreductasesOpen in Browse

CatLog read 127 records for EC 1.2.1.2 from 15 papers, for 12 organisms. Names in these papers: formate dehydrogenase (CboFDH), formate dehydrogenase, formate dehydrogenase (SoyFDH), W-formate dehydrogenase FdhAB (NvFdhAB) and 12 more.

EC 1.2.1.2 is no longer current; BRENDA: “transferred to EC 1.17.1.9”.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 1.2.1.2
Paper	Organisms	Records
Structure-guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP+	Candida boidinii	18
Functional effects of active site mutations in NAD+-dependent formate dehydrogenases on transformation of hydrogen carbonate to formate	Candida methylica, Chaetomium thermophilum	13
Insights into the catalytic mechanism of formate dehydrogenases from different microbial sources	Chaetomium thermophilum, Myceliophthora thermophila and 2 more	12
The role of ala198 in the stability and coenzyme specificity of bacterial formate dehydrogenases	Moraxella sp. C-1, Pseudomonas sp. 101	11
Structural Insights Into CO₂ Transport Pathways in a W-Formate Dehydrogenase: Structural Basis for CO₂ Reduction	Nitratidesulfovibrio vulgaris	10
doi:10.1016/j.molcatb.2013.05.020	Candida methylica	10
An unexpected alternative viologen electron mediator site in tungsten-containing formate dehydrogenase	Methylorubrum extorquens, Methylorubrum extorquens AM1	9
Engineering catalytic properties and thermal stability of plant formate dehydrogenase by single-point mutations	Glycine max	8
Identification of catalysis, substrate, and coenzyme binding sites and improvement catalytic efficiency of formate dehydrogenase from Candida boidinii	Candida boidinii	8
Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase	Candida boidinii	8
Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase	Paracoccus sp. 12-A	6
Additivity of the Stabilization Effect of Single Amino Acid Substitutions in Triple Mutants of Recombinant Formate Dehydrogenase from the Soybean Glycine max	Glycine max	6
doi:10.1016/j.procbio.2017.06.017	Myceliophthora thermophila	4
Promising properties of a formate dehydrogenase from a methanol-assimilating yeast Ogataea parapolymorpha DL-1 in His-tagged form	Ogataea parapolymorpha	2
Structural and Kinetic Studies of Formate Dehydrogenase from Candida boidinii	Candida boidinii	2

Records

Records for EC 1.2.1.2 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
CmFDH (NAD+-dependent formate dehydrogenase)	Candida methylica	formate	1.12	5.38	0.21	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase), formate
CmFDH (NAD+-dependent formate dehydrogenase)	Candida methylica	hydrogencarbonate	0.01	0.94	0.01	25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase), hydrogencarbonate
CmFDH (NAD+-dependent formate dehydrogenase)	Candida methylica	nicotinamide adenine dinucleotide (oxidized, NAD+)	0.91	0.69	1.31	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase), nicotinamide adenine dinucleotide (oxidized, NAD+)
CmFDH (NAD+-dependent formate dehydrogenase)variant H311D (His311Asp)	Candida methylica	formate	1.1	4.3	0.26	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant H311D (His311Asp), formate
CmFDH (NAD+-dependent formate dehydrogenase)variant H311D (His311Asp)	Candida methylica	nicotinamide adenine dinucleotide (oxidized, NAD+)	0.98	0.61	1.6	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant H311D (His311Asp), nicotinamide adenine dinucleotide (oxidized, NAD+)
CmFDH (NAD+-dependent formate dehydrogenase)variant N119H (Asn119His)	Candida methylica	formate	0.22	2.46	0.09	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119H (Asn119His), formate
CmFDH (NAD+-dependent formate dehydrogenase)variant N119H (Asn119His)	Candida methylica	nicotinamide adenine dinucleotide (oxidized, NAD+)	0.21	0.9	0.23	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119H (Asn119His), nicotinamide adenine dinucleotide (oxidized, NAD+)
CmFDH (NAD+-dependent formate dehydrogenase)variant N119Y (Asn119Tyr)	Candida methylica	formate	0.91	3.92	0.23	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119Y (Asn119Tyr), formate
CmFDH (NAD+-dependent formate dehydrogenase)variant N119Y (Asn119Tyr)	Candida methylica	nicotinamide adenine dinucleotide (oxidized, NAD+)	0.89	0.56	1.59	pH 8, 25 °C	PMID 30321426	Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119Y (Asn119Tyr), nicotinamide adenine dinucleotide (oxidized, NAD+)
CtFDH (NAD+-dependent formate dehydrogenase)	Chaetomium thermophilum	formate	1.805	3.7	0.49	pH 8, 25 °C	PMID 30321426	Open record: CtFDH (NAD+-dependent formate dehydrogenase), formate
CtFDH (NAD+-dependent formate dehydrogenase)	Chaetomium thermophilum	hydrogencarbonate	0.025	0.35	0.071	pH 8, 25 °C	PMID 30321426	Open record: CtFDH (NAD+-dependent formate dehydrogenase), hydrogencarbonate
CtFDH (NAD+-dependent formate dehydrogenase)variant N120C (Asn120Cys)	Chaetomium thermophilum	hydrogencarbonate	0.162	2.27	0.071	pH 7, 25 °C	PMID 30321426	Open record: CtFDH (NAD+-dependent formate dehydrogenase) variant N120C (Asn120Cys), hydrogencarbonate
CtFDH (NAD+-dependent formate dehydrogenase)variant N256D (Asn256Asp)	Chaetomium thermophilum	hydrogencarbonate	0.115	1.81	0.063	pH 8, 25 °C	PMID 30321426	Open record: CtFDH (NAD+-dependent formate dehydrogenase) variant N256D (Asn256Asp), hydrogencarbonate
formate dehydrogenase	Candida methylica	formate	1.13	4.75	0.24	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase, formate
formate dehydrogenasevariant H13E	Candida methylica	formate	0.55	5.3	0.1	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant H13E, formate
formate dehydrogenasevariant N147R	Candida methylica	formate	0.85	4.4	0.2	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant N147R, formate
formate dehydrogenasevariant N187E	Candida methylica	formate	0.7	3.9	0.18	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant N187E, formate
formate dehydrogenasevariant N187E/N147R	Candida methylica	formate	0.5	5.9	0.08	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant N187E/N147R, formate
formate dehydrogenasevariant N187E/Q105R	Candida methylica	formate	0.6	5.5	0.11	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant N187E/Q105R, formate
formate dehydrogenasevariant Q105R	Candida methylica	formate	1.2	5.1	0.24	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant Q105R, formate
formate dehydrogenasevariant Y160E	Candida methylica	formate	0.8	2.3	0.35	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant Y160E, formate
formate dehydrogenasevariant Y160R	Candida methylica	formate	0.3	2.7	0.11	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant Y160R, formate
formate dehydrogenasevariant Y302R	Candida methylica	formate	0.25	4.4	0.06	pH 8, 25 °C	doi:10.1016/j.molcatb.2013.05.020	Open record: formate dehydrogenase variant Y302R, formate
formate dehydrogenase	Ogataea parapolymorpha	NAD+	—	0.083	—	pH 6.5, 30 °C	PMID 23715855	Open record: formate dehydrogenase, NAD+
formate dehydrogenase	Ogataea parapolymorpha	sodium formate	2.32	0.82	2.83	pH 6.5, 30 °C	PMID 23715855	Open record: formate dehydrogenase, sodium formate
formate dehydrogenase	Paracoccus sp. 12-A	Formate	6.1	5.6	1.1	pH 7, 30 °C	PMID 15734738	Open record: formate dehydrogenase, Formate
formate dehydrogenase	Paracoccus sp. 12-A	Glyoxylate	0.007	7.5	9.3 × 10⁻⁴	pH 5.5, 30 °C	PMID 15734738	Open record: formate dehydrogenase, Glyoxylate
formate dehydrogenasevariant E141N	Paracoccus sp. 12-A	Formate	0.01	25	4 × 10⁻⁴	pH 7, 30 °C	PMID 15734738	Open record: formate dehydrogenase variant E141N, Formate
formate dehydrogenasevariant E141N	Paracoccus sp. 12-A	Glyoxylate	0.36	4.6	0.078	pH 5.5, 30 °C	PMID 15734738	Open record: formate dehydrogenase variant E141N, Glyoxylate
formate dehydrogenasevariant E141Q	Paracoccus sp. 12-A	Formate	0.054	31	0.0017	pH 7, 30 °C	PMID 15734738	Open record: formate dehydrogenase variant E141Q, Formate
formate dehydrogenasevariant E141Q	Paracoccus sp. 12-A	Glyoxylate	0.023	2.6	0.0088	pH 5.5, 30 °C	PMID 15734738	Open record: formate dehydrogenase variant E141Q, Glyoxylate
formate dehydrogenase (CbFDH_iso2)	Candida boidinii	formate	—	4	1.4	pH 7.5, 25 °C	PMID 27100912	Open record: formate dehydrogenase (CbFDH_iso2), formate
formate dehydrogenase (CbFDH_iso2)	Candida boidinii	NAD+	5.6	0.0303	180	pH 7.5, 25 °C	PMID 27100912	Open record: formate dehydrogenase (CbFDH_iso2), NAD+
formate dehydrogenase (CboFDH)	Candida boidinii	ammonium formate	1.534	3.63	0.423	pH 9.5	PMID 27198726	Open record: formate dehydrogenase (CboFDH), ammonium formate
formate dehydrogenase (CboFDH)	Candida boidinii	NAD+	3.7	0.015	246.7	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH), NAD+
formate dehydrogenase (CboFDH)	Candida boidinii	NAD+	22.30	3.4	6.618	pH 9.5	PMID 27198726	Open record: formate dehydrogenase (CboFDH), NAD+
formate dehydrogenase (CboFDH)	Candida boidinii	NADP+	4 × 10⁻⁵	—	—	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH), NADP+
formate dehydrogenase (CboFDH)variant D195A	Candida boidinii	NAD+	0.76	4.8	0.158	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195A, NAD+
formate dehydrogenase (CboFDH)variant D195A	Candida boidinii	NADP+	0.052	3.3	0.0157	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195A, NADP+
formate dehydrogenase (CboFDH)variant D195N	Candida boidinii	NAD+	0.21	5.01	0.0419	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195N, NAD+
formate dehydrogenase (CboFDH)variant D195N	Candida boidinii	NADP+	0.26	13.2	0.0196	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195N, NADP+
formate dehydrogenase (CboFDH)variant D195Q	Candida boidinii	NAD+	0.26	0.96	0.271	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q, NAD+
formate dehydrogenase (CboFDH)variant D195Q	Candida boidinii	NADP+	0.26	4.5	0.058	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q, NADP+
formate dehydrogenase (CboFDH)variant D195Q/Y196H	Candida boidinii	formatecosubstrate NAD+	—	139.6	—	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196H, formate
formate dehydrogenase (CboFDH)variant D195Q/Y196H	Candida boidinii	formatecosubstrate NADP+	—	80.2	—	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196H, formate
formate dehydrogenase (CboFDH)variant D195Q/Y196H	Candida boidinii	NAD+	0.49	1.8	0.27	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196H, NAD+
formate dehydrogenase (CboFDH)variant D195Q/Y196H	Candida boidinii	NADP+	0.44	1.7	0.26	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196H, NADP+
formate dehydrogenase (CboFDH)variant D195Q/Y196P	Candida boidinii	NAD+	0.87	0.13	6.69	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196P, NAD+
formate dehydrogenase (CboFDH)variant D195Q/Y196P	Candida boidinii	NADP+	0.34	3.7	0.092	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196P, NADP+
formate dehydrogenase (CboFDH)variant D195Q/Y196S	Candida boidinii	NAD+	0.4	5.1	0.078	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196S, NAD+
formate dehydrogenase (CboFDH)variant D195Q/Y196S	Candida boidinii	NADP+	0.34	6.2	0.055	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195Q/Y196S, NADP+
formate dehydrogenase (CboFDH)variant D195S	Candida boidinii	NAD+	0.34	1.5	0.227	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195S, NAD+
formate dehydrogenase (CboFDH)variant D195S	Candida boidinii	NADP+	0.34	6.2	0.055	pH 7.5	PMID 18616465	Open record: formate dehydrogenase (CboFDH) variant D195S, NADP+
formate dehydrogenase (CboFDH)variant N187D	Candida boidinii	ammonium formate	3.285	6.7	0.491	pH 10	PMID 27198726	Open record: formate dehydrogenase (CboFDH) variant N187D, ammonium formate
formate dehydrogenase (CboFDH)variant N187D	Candida boidinii	NAD+	28.99	3.8	9.808	pH 10	PMID 27198726	Open record: formate dehydrogenase (CboFDH) variant N187D, NAD+
formate dehydrogenase (CboFDH)variant V120S	Candida boidinii	ammonium formate	5.335	7.9	0.6758	pH 9.5	PMID 27198726	Open record: formate dehydrogenase (CboFDH) variant V120S, ammonium formate
formate dehydrogenase (CboFDH)variant V120S	Candida boidinii	NAD+	40.6	8.81	5.008	pH 9.5	PMID 27198726	Open record: formate dehydrogenase (CboFDH) variant V120S, NAD+
formate dehydrogenase (CboFDH)variant V120S/N187D	Candida boidinii	ammonium formate	0.6967	2.5	0.2752	pH 9.5	PMID 27198726	Open record: formate dehydrogenase (CboFDH) variant V120S/N187D, ammonium formate
formate dehydrogenase (CboFDH)variant V120S/N187D	Candida boidinii	NAD+	18.68	2.02	9.934	pH 9.5	PMID 27198726	Open record: formate dehydrogenase (CboFDH) variant V120S/N187D, NAD+
formate dehydrogenase (ctFDH)	Chaetomium thermophilum	formate	1.92	6.19	0.31	pH 5, 25 °C	PMID 41784227	Open record: formate dehydrogenase (ctFDH), formate
formate dehydrogenase (ctFDH)	Chaetomium thermophilum	formate	1.22	2.18	0.56	pH 6, 25 °C	PMID 41784227	Open record: formate dehydrogenase (ctFDH), formate
formate dehydrogenase (ctFDH)	Chaetomium thermophilum	formate	0.64	1.41	0.45	pH 8, 25 °C	PMID 41784227	Open record: formate dehydrogenase (ctFDH), formate
formate dehydrogenase (MorFDH)variant A198G	Moraxella sp. C-1	formate	—	8	—	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (MorFDH) variant A198G, formate
formate dehydrogenase (MorFDH)variant A198G	Moraxella sp. C-1	NAD+	7.3	0.045	162	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (MorFDH) variant A198G, NAD+
formate dehydrogenase (mtFDH)	Myceliophthora thermophila	formate	6.62	2.85	2.32	pH 5, 25 °C	PMID 41784227	Open record: formate dehydrogenase (mtFDH), formate
formate dehydrogenase (mtFDH)	Myceliophthora thermophila	formate	5.73	1.88	3.04	pH 6, 25 °C	PMID 41784227	Open record: formate dehydrogenase (mtFDH), formate
formate dehydrogenase (mtFDH)	Myceliophthora thermophila	formate	5.35	1.39	3.85	pH 8, 25 °C	PMID 41784227	Open record: formate dehydrogenase (mtFDH), formate
formate dehydrogenase (opFDH)	Ogataea parapolymorpha	formate	0.25	7.58	0.03	pH 5, 25 °C	PMID 41784227	Open record: formate dehydrogenase (opFDH), formate
formate dehydrogenase (opFDH)	Ogataea parapolymorpha	formate	0.13	3.27	0.04	pH 6, 25 °C	PMID 41784227	Open record: formate dehydrogenase (opFDH), formate
formate dehydrogenase (opFDH)	Ogataea parapolymorpha	formate	0.18	1.74	0.1	pH 8, 25 °C	PMID 41784227	Open record: formate dehydrogenase (opFDH), formate
formate dehydrogenase (pseFDH)	Pseudomonas sp. 101	formate	5.56	4.65	1.2	pH 5, 25 °C	PMID 41784227	Open record: formate dehydrogenase (pseFDH), formate
formate dehydrogenase (pseFDH)	Pseudomonas sp. 101	formate	4.45	3.78	1.17	pH 6, 25 °C	PMID 41784227	Open record: formate dehydrogenase (pseFDH), formate
formate dehydrogenase (pseFDH)	Pseudomonas sp. 101	formate	5.48	5.78	0.95	pH 8, 25 °C	PMID 41784227	Open record: formate dehydrogenase (pseFDH), formate
formate dehydrogenase (PseFDH)	Pseudomonas sp. 101	NADP+	1.3	100	0.013	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH), NADP+
formate dehydrogenase (PseFDH)variant A198G	Pseudomonas sp. 101	formate	—	7.5	—	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant A198G, formate
formate dehydrogenase (PseFDH)variant A198G	Pseudomonas sp. 101	NAD+	7.3	0.035	209	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant A198G, NAD+
formate dehydrogenase (PseFDH)variant D221S	Pseudomonas sp. 101	formate	—	32	—	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant D221S, formate
formate dehydrogenase (PseFDH)variant D221S	Pseudomonas sp. 101	NAD+	5	0.71	7.04	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant D221S, NAD+
formate dehydrogenase (PseFDH)variant D221S/A198G	Pseudomonas sp. 101	formatecosubstrate NAD+	—	53	—	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant D221S/A198G, formate
formate dehydrogenase (PseFDH)variant D221S/A198G	Pseudomonas sp. 101	formatecosubstrate NADP+	—	89	—	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant D221S/A198G, formate
formate dehydrogenase (PseFDH)variant D221S/A198G	Pseudomonas sp. 101	NAD+	5	0.54	9.26	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant D221S/A198G, NAD+
formate dehydrogenase (PseFDH)variant D221S/A198G	Pseudomonas sp. 101	NADP+	1.8	0.28	6.43	pH 7, 30 °C	PMID 25927002	Open record: formate dehydrogenase (PseFDH) variant D221S/A198G, NADP+
formate dehydrogenase (SoyFDH)	Glycine max	formate	2.9	1.5	1.93	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH), formate
formate dehydrogenase (SoyFDH)	Glycine max	NAD+	—	0.0133	220	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH), NAD+
formate dehydrogenase (SoyFDH)variant A267M/I272V/F290D	Glycine max	nicotinamide adenine dinucleotide (oxidized)	2.9	0.0203	140	pH 7, 30 °C	PMID 26483960	Open record: formate dehydrogenase (SoyFDH) variant A267M/I272V/F290D, nicotinamide adenine dinucleotide (oxidized)
formate dehydrogenase (SoyFDH)variant A267M/I272V/F290D	Glycine max	sodium formate	—	2.8	1.04	pH 7, 30 °C	PMID 26483960	Open record: formate dehydrogenase (SoyFDH) variant A267M/I272V/F290D, sodium formate
formate dehydrogenase (SoyFDH)variant A267M/I272V/F290N	Glycine max	nicotinamide adenine dinucleotide (oxidized)	3.2	0.0141	230	pH 7, 30 °C	PMID 26483960	Open record: formate dehydrogenase (SoyFDH) variant A267M/I272V/F290N, nicotinamide adenine dinucleotide (oxidized)
formate dehydrogenase (SoyFDH)variant A267M/I272V/F290N	Glycine max	sodium formate	—	2.2	1.45	pH 7, 30 °C	PMID 26483960	Open record: formate dehydrogenase (SoyFDH) variant A267M/I272V/F290N, sodium formate
formate dehydrogenase (SoyFDH)variant A267M/I272V/F290S	Glycine max	nicotinamide adenine dinucleotide (oxidized)	3.7	0.0161	230	pH 7, 30 °C	PMID 26483960	Open record: formate dehydrogenase (SoyFDH) variant A267M/I272V/F290S, nicotinamide adenine dinucleotide (oxidized)
formate dehydrogenase (SoyFDH)variant A267M/I272V/F290S	Glycine max	sodium formate	—	2.3	1.61	pH 7, 30 °C	PMID 26483960	Open record: formate dehydrogenase (SoyFDH) variant A267M/I272V/F290S, sodium formate
formate dehydrogenase (SoyFDH)variant F290D	Glycine max	formate	5.1	5	1.02	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH) variant F290D, formate
formate dehydrogenase (SoyFDH)variant F290D	Glycine max	NAD+	—	0.0128	400	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH) variant F290D, NAD+
formate dehydrogenase (SoyFDH)variant F290N	Glycine max	formate	2.8	4.5	0.62	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH) variant F290N, formate
formate dehydrogenase (SoyFDH)variant F290N	Glycine max	NAD+	—	0.014	200	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH) variant F290N, NAD+
formate dehydrogenase (SoyFDH)variant F290S	Glycine max	formate	4.1	4.1	1	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH) variant F290S, formate
formate dehydrogenase (SoyFDH)variant F290S	Glycine max	NAD+	—	0.0091	450	pH 7, 30 °C	PMID 23100543	Open record: formate dehydrogenase (SoyFDH) variant F290S, NAD+
formate dehydrogenase 1 (MeFDH1)variant F232A/F471A/Y329A	Methylorubrum extorquens	ethyl viologen	179.4	2.54	70.65	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant F232A/F471A/Y329A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)	Methylorubrum extorquens AM1	ethyl viologen	715.0	0.91	785.8	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1), ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237A	Methylorubrum extorquens AM1	ethyl viologen	179.8	1.16	155.0	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237A/F232A	Methylorubrum extorquens AM1	ethyl viologen	202	1.74	116.3	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237A/F232A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237A/F232A/F471A/Y329A	Methylorubrum extorquens AM1	ethyl viologen	69.01	3.13	22.05	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237A/F232A/F471A/Y329A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237A/F471A	Methylorubrum extorquens AM1	ethyl viologen	136.1	1.97	69.09	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237A/F471A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237A/F471A/Y329A	Methylorubrum extorquens AM1	ethyl viologen	72.8	3.28	22.2	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237A/F471A/Y329A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237A/Y329A	Methylorubrum extorquens AM1	ethyl viologen	95.5	2.78	34.35	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237A/Y329A, ethyl viologen
formate dehydrogenase 1 (MeFDH1)variant K237E	Methylorubrum extorquens AM1	ethyl viologen	162.8	1.07	152.2	pH 6.3, 30 °C	PMID 42206755	Open record: formate dehydrogenase 1 (MeFDH1) variant K237E, ethyl viologen
NAD+-dependent formate dehydrogenase (CboFDH)	Candida boidinii	formate	—	7.3	—	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH), formate
NAD+-dependent formate dehydrogenase (CboFDH)	Candida boidinii	nicotinamide adenine dinucleotide (oxidized)	3.3	0.0536	62	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH), nicotinamide adenine dinucleotide (oxidized)
NAD+-dependent formate dehydrogenase (CboFDH)variant A10C	Candida boidinii	formate	—	8.2	—	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH) variant A10C, formate
NAD+-dependent formate dehydrogenase (CboFDH)variant A10C	Candida boidinii	nicotinamide adenine dinucleotide (oxidized)	6.2	0.0742	84	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH) variant A10C, nicotinamide adenine dinucleotide (oxidized)
NAD+-dependent formate dehydrogenase (CboFDH)variant A10C/I239C	Candida boidinii	formate	—	5.8	—	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH) variant A10C/I239C, formate
NAD+-dependent formate dehydrogenase (CboFDH)variant A10C/I239C	Candida boidinii	nicotinamide adenine dinucleotide (oxidized)	4.3	0.1191	36	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH) variant A10C/I239C, nicotinamide adenine dinucleotide (oxidized)
NAD+-dependent formate dehydrogenase (CboFDH)variant I239C	Candida boidinii	formate	—	5.2	—	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH) variant I239C, formate
NAD+-dependent formate dehydrogenase (CboFDH)variant I239C	Candida boidinii	nicotinamide adenine dinucleotide (oxidized)	2.6	0.1322	20	pH 7, 30 °C	PMID 27836850	Open record: NAD+-dependent formate dehydrogenase (CboFDH) variant I239C, nicotinamide adenine dinucleotide (oxidized)
NAD+-dependent formate dehydrogenase (MtFDH)	Myceliophthora thermophila	NAD+	0.76	1.2	0.63	pH 10.5, 25 °C	doi:10.1016/j.procbio.2017.06.017	Open record: NAD+-dependent formate dehydrogenase (MtFDH), NAD+
NAD+-dependent formate dehydrogenase (MtFDH)	Myceliophthora thermophila	NADH	0.03	0.18	0.17	25 °C	doi:10.1016/j.procbio.2017.06.017	Open record: NAD+-dependent formate dehydrogenase (MtFDH), NADH
NAD+-dependent formate dehydrogenase (MtFDH)	Myceliophthora thermophila	sodium bicarbonate	0.1	0.43	0.23	25 °C	doi:10.1016/j.procbio.2017.06.017	Open record: NAD+-dependent formate dehydrogenase (MtFDH), sodium bicarbonate
NAD+-dependent formate dehydrogenase (MtFDH)	Myceliophthora thermophila	sodium formate	0.32	7.2	0.04	pH 10.5, 25 °C	doi:10.1016/j.procbio.2017.06.017	Open record: NAD+-dependent formate dehydrogenase (MtFDH), sodium formate
W-formate dehydrogenase FdhAB (NvFdhAB)variant H457R	Nitratidesulfovibrio vulgaris	carbon dioxide	80	0.49	163		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant H457R, carbon dioxide
W-formate dehydrogenase FdhAB (NvFdhAB)variant H457R	Nitratidesulfovibrio vulgaris	formate	330	0.07	4459		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant H457R, formate
W-formate dehydrogenase FdhAB (NvFdhAB)variant Q447A	Nitratidesulfovibrio vulgaris	carbon dioxide	23	5.8	4		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant Q447A, carbon dioxide
W-formate dehydrogenase FdhAB (NvFdhAB)variant Q447A	Nitratidesulfovibrio vulgaris	formate	370	0.067	5522		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant Q447A, formate
W-formate dehydrogenase FdhAB (NvFdhAB)variant S194A	Nitratidesulfovibrio vulgaris	carbon dioxide	60	0.68	88		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant S194A, carbon dioxide
W-formate dehydrogenase FdhAB (NvFdhAB)variant S194A	Nitratidesulfovibrio vulgaris	formate	220	0.79	278		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant S194A, formate
W-formate dehydrogenase FdhAB (NvFdhAB)variant V197S	Nitratidesulfovibrio vulgaris	carbon dioxide	11	5.8	2		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant V197S, carbon dioxide
W-formate dehydrogenase FdhAB (NvFdhAB)variant V197S	Nitratidesulfovibrio vulgaris	formate	1.08 × 10³	0.314	3439		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant V197S, formate
W-formate dehydrogenase FdhAB (NvFdhAB)variant W491E	Nitratidesulfovibrio vulgaris	carbon dioxide	230	11.6	20		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant W491E, carbon dioxide
W-formate dehydrogenase FdhAB (NvFdhAB)variant W491E	Nitratidesulfovibrio vulgaris	formate	1.18 × 10³	0.12	9833		PMID 41787858	Open record: W-formate dehydrogenase FdhAB (NvFdhAB) variant W491E, formate