Values CatLog read from this paper
Functional effects of active site mutations in NAD+-dependent formate dehydrogenases on transformation of hydrogen carbonate to formate
PMID 30321426doi:10.1093/protein/gzy027Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (39 values) from this paper: CmFDH (NAD+-dependent formate dehydrogenase), CtFDH (NAD+-dependent formate dehydrogenase) from Candida methylica, Chaetomium thermophilum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CmFDH (NAD+-dependent formate dehydrogenase) | Candida methylica | formate | 1.12 | 5.38 | 0.21 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase), formate |
| CmFDH (NAD+-dependent formate dehydrogenase) | Candida methylica | hydrogencarbonate | 0.01 | 0.94 | 0.01 | 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase), hydrogencarbonate |
| CmFDH (NAD+-dependent formate dehydrogenase) | Candida methylica | nicotinamide adenine dinucleotide (oxidized, NAD+) | 0.91 | 0.69 | 1.31 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase), nicotinamide adenine dinucleotide (oxidized, NAD+) |
| CmFDH (NAD+-dependent formate dehydrogenase)variant H311D (His311Asp) | Candida methylica | formate | 1.1 | 4.3 | 0.26 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant H311D (His311Asp), formate |
| CmFDH (NAD+-dependent formate dehydrogenase)variant H311D (His311Asp) | Candida methylica | nicotinamide adenine dinucleotide (oxidized, NAD+) | 0.98 | 0.61 | 1.6 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant H311D (His311Asp), nicotinamide adenine dinucleotide (oxidized, NAD+) |
| CmFDH (NAD+-dependent formate dehydrogenase)variant N119H (Asn119His) | Candida methylica | formate | 0.22 | 2.46 | 0.09 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119H (Asn119His), formate |
| CmFDH (NAD+-dependent formate dehydrogenase)variant N119H (Asn119His) | Candida methylica | nicotinamide adenine dinucleotide (oxidized, NAD+) | 0.21 | 0.9 | 0.23 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119H (Asn119His), nicotinamide adenine dinucleotide (oxidized, NAD+) |
| CmFDH (NAD+-dependent formate dehydrogenase)variant N119Y (Asn119Tyr) | Candida methylica | formate | 0.91 | 3.92 | 0.23 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119Y (Asn119Tyr), formate |
| CmFDH (NAD+-dependent formate dehydrogenase)variant N119Y (Asn119Tyr) | Candida methylica | nicotinamide adenine dinucleotide (oxidized, NAD+) | 0.89 | 0.56 | 1.59 | pH 8, 25 °C | Open record: CmFDH (NAD+-dependent formate dehydrogenase) variant N119Y (Asn119Tyr), nicotinamide adenine dinucleotide (oxidized, NAD+) |
| CtFDH (NAD+-dependent formate dehydrogenase) | Chaetomium thermophilum | formate | 1.805 | 3.7 | 0.49 | pH 8, 25 °C | Open record: CtFDH (NAD+-dependent formate dehydrogenase), formate |
| CtFDH (NAD+-dependent formate dehydrogenase) | Chaetomium thermophilum | hydrogencarbonate | 0.025 | 0.35 | 0.071 | pH 8, 25 °C | Open record: CtFDH (NAD+-dependent formate dehydrogenase), hydrogencarbonate |
| CtFDH (NAD+-dependent formate dehydrogenase)variant N120C (Asn120Cys) | Chaetomium thermophilum | hydrogencarbonate | 0.162 | 2.27 | 0.071 | pH 7, 25 °C | Open record: CtFDH (NAD+-dependent formate dehydrogenase) variant N120C (Asn120Cys), hydrogencarbonate |
| CtFDH (NAD+-dependent formate dehydrogenase)variant N256D (Asn256Asp) | Chaetomium thermophilum | hydrogencarbonate | 0.115 | 1.81 | 0.063 | pH 8, 25 °C | Open record: CtFDH (NAD+-dependent formate dehydrogenase) variant N256D (Asn256Asp), hydrogencarbonate |