CatLogenzyme kinetics catalog

EC 1.2.3.3: pyruvate oxidase

OxidoreductasesOpen in Browse

CatLog read 8 records for EC 1.2.3.3 from 1 paper, for 1 organism. Names in these papers: pyruvate oxidase (POX).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 7 records for EC 1.2.3.3 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.2.3.3 in Browse.

Papers

PaperOrganismsRecords
Activation of thiamin diphosphate and FAD in the phosphatedependent pyruvate oxidase from Lactobacillus plantarumLactobacillus plantarum8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
pyruvate oxidase (POX)Lactobacillus plantarum2,6-dichlorophenolindophenolDCPIP assay (DCPIP reduction followed at 600 nm)—0.02—pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarum2,6-dichlorophenolindophenol40 mM pyruvate—0.024—pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarumdioxygen—0.07—pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarumphosphateDCPIP assay (DCPIP reduction followed at 600 nm)—4.5—pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarumphosphatecoupled assay: H2O2 detected with 4-aminoantipyrine (2 mM)—2.3—pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarumpyruvateDCPIP assay (DCPIP reduction followed at 600 nm)17.91.611 calc.pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarumpyruvatecoupled assay: H2O2 detected with 4-aminoantipyrine (2 mM)9.60.424 calc.pH 6, 25 °CPMID 9582325Open
pyruvate oxidase (POX)Lactobacillus plantarumpyruvate2——pH 6, 4 °CPMID 9582325Open