CatLogenzyme kinetics catalog

Values CatLog read from this paper

Activation of thiamin diphosphate and FAD in the phosphatedependent pyruvate oxidase from Lactobacillus plantarum

PMID 9582325doi:10.1074/jbc.273.21.12929Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (10 values) from this paper: pyruvate oxidase (POX) from Lactobacillus plantarum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.2.3.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
pyruvate oxidase (POX)Lactobacillus plantarum2,6-dichlorophenolindophenolDCPIP assay (DCPIP reduction followed at 600 nm)—0.02—pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarum2,6-dichlorophenolindophenol40 mM pyruvate—0.024—pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarumdioxygen—0.07—pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarumphosphateDCPIP assay (DCPIP reduction followed at 600 nm)—4.5—pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarumphosphatecoupled assay: H2O2 detected with 4-aminoantipyrine (2 mM)—2.3—pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarumpyruvateDCPIP assay (DCPIP reduction followed at 600 nm)17.91.611 calc.pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarumpyruvatecoupled assay: H2O2 detected with 4-aminoantipyrine (2 mM)9.60.424 calc.pH 6, 25 °COpen
pyruvate oxidase (POX)Lactobacillus plantarumpyruvate2——pH 6, 4 °COpen