CatLogenzyme kinetics catalog

EC 2.1.1.220: tRNA (adenine58-N1)-methyltransferase

TransferasesOpen in Browse

CatLog read 8 records for EC 2.1.1.220 from 1 paper, for 1 organism. Names in these papers: tRNA m1A58 methyltransferase (thTrmI).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 3 records for EC 2.1.1.220 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.220 in Browse.

Papers

PaperOrganismsRecords
Crystal structure of Thermus thermophilus tRNA m1A58 methyltransferase and biophysical characterization of its interaction with tRNAThermus thermophilus8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
tRNA m1A58 methyltransferase (thTrmI)Thermus thermophilusEscherichia coli initiator tRNA(Met)0.0360.002713 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)Thermus thermophilusS-adenosyl-L-methionine0.0370.002118 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)variant D170AThermus thermophilusEscherichia coli initiator tRNA(Met)1.1 × 10⁻⁴1.7 × 10⁻⁴0.65 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)variant D170AThermus thermophilusS-adenosyl-L-methionine1.3 × 10⁻⁴0.00540.024 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)variant Y194AThermus thermophilusEscherichia coli initiator tRNA(Met)0.010.00224.5 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)variant Y194AThermus thermophilusS-adenosyl-L-methionine0.0060.00571.1 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)variant Y78AThermus thermophilusEscherichia coli initiator tRNA(Met)0.00161.6 × 10⁻⁴10 calc.pH 8, 60 °CPMID 18262540Open
tRNA m1A58 methyltransferase (thTrmI)variant Y78AThermus thermophilusS-adenosyl-L-methionine0.00140.00190.74 calc.pH 8, 60 °CPMID 18262540Open