CatLogenzyme kinetics catalog

Values CatLog read from this paper

Crystal structure of Thermus thermophilus tRNA m1A58 methyltransferase and biophysical characterization of its interaction with tRNA

PMID 18262540doi:10.1016/j.jmb.2008.01.041Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (16 values) from this paper: tRNA m1A58 methyltransferase (thTrmI) from Thermus thermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.220

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
tRNA m1A58 methyltransferase (thTrmI)Thermus thermophilusEscherichia coli initiator tRNA(Met)0.0360.002713 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)Thermus thermophilusS-adenosyl-L-methionine0.0370.002118 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)variant D170AThermus thermophilusEscherichia coli initiator tRNA(Met)1.1 × 10⁻⁴1.7 × 10⁻⁴0.65 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)variant D170AThermus thermophilusS-adenosyl-L-methionine1.3 × 10⁻⁴0.00540.024 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)variant Y194AThermus thermophilusEscherichia coli initiator tRNA(Met)0.010.00224.5 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)variant Y194AThermus thermophilusS-adenosyl-L-methionine0.0060.00571.1 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)variant Y78AThermus thermophilusEscherichia coli initiator tRNA(Met)0.00161.6 × 10⁻⁴10 calc.pH 8, 60 °COpen
tRNA m1A58 methyltransferase (thTrmI)variant Y78AThermus thermophilusS-adenosyl-L-methionine0.00140.00190.74 calc.pH 8, 60 °COpen