EC 2.1.1.85: protein-histidine N-methyltransferase
TransferasesOpen in Browse
CatLog read 42 records for EC 2.1.1.85 from 5 papers, for 2 organisms. Names in these papers: SETD3 (actin histidine N-methyltransferase), SETD3 (histidine N3-methyltransferase), SETD3 (actin histidine N-tau-methyltransferase), SETD3 (actin-specific histidine N-methyltransferase) and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 2 records for EC 2.1.1.85 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.85 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Structural insights into SETD3-mediated histidine methylation on β-actin | Homo sapiens | 18 |
| Importance of Ile71 in β-actin on histidine methyltransferase SETD3 catalysis | Homo sapiens | 9 |
| SETD3 protein is the actin-specific histidine N-methyltransferase | Homo sapiens, Rattus norvegicus | 6 |
| Histidine methyltransferase SETD3 methylates structurally diverse histidine mimics in actin | Homo sapiens | 6 |
| Histidine Ethylation by Histidine Methyltransferases SETD3 and METTL9 | Homo sapiens | 3 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| SETD3 | Homo sapiens | S-adenosylethionine | 0.037 | 0.0062 | 6.7 | pH 9, 37 °C | PMID 42307994 | Open record: SETD3, S-adenosylethionine |
| SETD3 | Homo sapiens | S-adenosylmethionine | 2.87 | 0.0047 | 605 | pH 9, 37 °C | PMID 42307994 | Open record: SETD3, S-adenosylmethionine |
| SETD3 | Homo sapiens | Se-adenosylselenoethionine | 1.003 | 0.0036 | 275 | pH 9, 37 °C | PMID 42307994 | Open record: SETD3, Se-adenosylselenoethionine |
| SETD3 (actin histidine N-methyltransferase) | Homo sapiens | S-adenosyl-L-methionine | 0.01122 | 1.11 × 10⁻⁴ | 101.0 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase), S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase) | Homo sapiens | β-actin | 0.01348 | 5.02 × 10⁻⁴ | 26.87 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase), β-actin |
| SETD3 (actin histidine N-methyltransferase)variant N256A | Homo sapiens | S-adenosyl-L-methionine | 0.00113 | 6.86 × 10⁻⁴ | 1.65 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N256A, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant N256A | Homo sapiens | β-actin | 5.2 × 10⁻⁴ | 4.49 × 10⁻⁴ | 1.15 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N256A, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant N256D | Homo sapiens | S-adenosyl-L-methionine | 6.7 × 10⁻⁴ | 1.65 × 10⁻⁴ | 4.03 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N256D, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant N256D | Homo sapiens | β-actin | 9.7 × 10⁻⁴ | 3.05 × 10⁻⁴ | 3.17 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N256D, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant N256Q | Homo sapiens | S-adenosyl-L-methionine | 0.00633 | 1.03 × 10⁻⁴ | 61.48 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N256Q, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant N256Q | Homo sapiens | β-actin | 0.01082 | 7.84 × 10⁻⁴ | 13.8 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N256Q, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant N278A | Homo sapiens | S-adenosyl-L-methionine | 1.7 × 10⁻⁵ | 3.5 × 10⁻⁴ | 0.05 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N278A, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant N278A | Homo sapiens | β-actin | 1.7 × 10⁻⁵ | 4.7 × 10⁻⁵ | 0.35 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant N278A, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant R215A | Homo sapiens | S-adenosyl-L-methionine | 0.00365 | 1.65 × 10⁻⁴ | 22.12 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant R215A, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant R215A | Homo sapiens | β-actin | 0.00353 | 0.001087 | 3.25 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant R215A, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant R316A | Homo sapiens | S-adenosyl-L-methionine | 0.00407 | 1.49 × 10⁻⁴ | 27.3 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant R316A, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant R316A | Homo sapiens | β-actin | 0.00288 | 0.001115 | 2.58 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant R316A, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant R75A | Homo sapiens | S-adenosyl-L-methionine | 2.7 × 10⁻⁴ | 0.003601 | 0.067 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant R75A, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant R75A | Homo sapiens | β-actin | 3.3 × 10⁻⁵ | 5.65 × 10⁻⁴ | 0.067 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant R75A, β-actin |
| SETD3 (actin histidine N-methyltransferase)variant Y313F | Homo sapiens | S-adenosyl-L-methionine | 0.00162 | 0.006543 | 0.25 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant Y313F, S-adenosyl-L-methionine |
| SETD3 (actin histidine N-methyltransferase)variant Y313F | Homo sapiens | β-actin | 3.7 × 10⁻⁴ | 5.81 × 10⁻⁴ | 0.63 | pH 7.2, 37 °C | PMID 30785395 | Open record: SETD3 (actin histidine N-methyltransferase) variant Y313F, β-actin |
| SETD3 (actin histidine N-tau-methyltransferase) | Homo sapiens | 16-mer β-actin peptide (βA residues 66-81) with 3-pyridylalanine at position 73 | 0.813 | 0.0629 | 13 | pH 9, 37 °C | PMID 35481649 | Open record: SETD3 (actin histidine N-tau-methyltransferase), 16-mer β-actin peptide (βA residues 66-81) with 3-pyridylalanine at position 73 |
| SETD3 (actin histidine N-tau-methyltransferase) | Homo sapiens | 16-mer β-actin peptide (βA residues 66-81) with 4-pyridylalanine at position 73 | 0.793 | 0.0338 | 23.5 | pH 9, 37 °C | PMID 35481649 | Open record: SETD3 (actin histidine N-tau-methyltransferase), 16-mer β-actin peptide (βA residues 66-81) with 4-pyridylalanine at position 73 |
| SETD3 (actin histidine N-tau-methyltransferase) | Homo sapiens | 16-mer β-actin peptide (βA residues 66-81) with L-histidine at position 73 | 2.35 | 0.0336 | 70.3 | pH 9, 37 °C | PMID 35481649 | Open record: SETD3 (actin histidine N-tau-methyltransferase), 16-mer β-actin peptide (βA residues 66-81) with L-histidine at position 73 |
| SETD3 (actin histidine N-tau-methyltransferase) | Homo sapiens | 16-mer β-actin peptide (βA residues 66-81) with N(pi)-methyl-histidine at position 73 | 0.47 | 0.0886 | 5.3 | pH 9, 37 °C | PMID 35481649 | Open record: SETD3 (actin histidine N-tau-methyltransferase), 16-mer β-actin peptide (βA residues 66-81) with N(pi)-methyl-histidine at position 73 |
| SETD3 (actin histidine N-tau-methyltransferase) | Homo sapiens | 16-mer β-actin peptide (βA residues 66-81) with ornithine at position 73 | 0.233 | 0.0958 | 2.5 | pH 9, 37 °C | PMID 35481649 | Open record: SETD3 (actin histidine N-tau-methyltransferase), 16-mer β-actin peptide (βA residues 66-81) with ornithine at position 73 |
| SETD3 (actin histidine N-tau-methyltransferase) | Homo sapiens | 16-mer β-actin peptide (βA residues 66-81) with triazol-4-yl-alanine at position 73 | 2.3 | 0.0667 | 34.5 | pH 9, 37 °C | PMID 35481649 | Open record: SETD3 (actin histidine N-tau-methyltransferase), 16-mer β-actin peptide (βA residues 66-81) with triazol-4-yl-alanine at position 73 |
| SETD3 (actin-specific histidine N-methyltransferase) | Homo sapiens | recombinant human beta-actin (E. coli-produced, nucleotide-free) | 0.0108 | 7.52 × 10⁻⁴ | 14.3 | pH 7.2, 37 °C | PMID 30526847 | Open record: SETD3 (actin-specific histidine N-methyltransferase), recombinant human beta-actin (E. coli-produced, nucleotide-free) |
| SETD3 (actin-specific histidine N-methyltransferase) | Homo sapiens | S-adenosyl-L-methionine | 0.0102 | 1.16 × 10⁻⁴ | 87.5 | pH 7.2, 37 °C | PMID 30526847 | Open record: SETD3 (actin-specific histidine N-methyltransferase), S-adenosyl-L-methionine |
| SETD3 (actin-specific histidine N-methyltransferase) | Homo sapiens | YPIEHGIVT | 3.3 × 10⁻⁵ | 8.729 | 3.8 × 10⁻⁶ | pH 7.2, 37 °C | PMID 30526847 | Open record: SETD3 (actin-specific histidine N-methyltransferase), YPIEHGIVT |
| SETD3 (actin-specific histidine N-methyltransferase) | Rattus norvegicus | recombinant human beta-actin (E. coli-produced, nucleotide-free) | 0.0133 | 0.002996 | 4.5 | pH 7.2, 37 °C | PMID 30526847 | Open record: SETD3 (actin-specific histidine N-methyltransferase), recombinant human beta-actin (E. coli-produced, nucleotide-free) |
| SETD3 (actin-specific histidine N-methyltransferase) | Rattus norvegicus | S-adenosyl-L-methionine | 0.0095 | 1.09 × 10⁻⁴ | 87.2 | pH 7.2, 37 °C | PMID 30526847 | Open record: SETD3 (actin-specific histidine N-methyltransferase), S-adenosyl-L-methionine |
| SETD3 (actin-specific histidine N-methyltransferase) | Rattus norvegicus | YPIEHGIVT | 8.3 × 10⁻⁵ | 10.59 | 7.8 × 10⁻⁶ | pH 7.2, 37 °C | PMID 30526847 | Open record: SETD3 (actin-specific histidine N-methyltransferase), YPIEHGIVT |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by 2-aminobutyric acid | 1.353 | 0.0337 | 40.2 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by 2-aminobutyric acid |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by 2-aminoheptanoic acid | 2.37 | 0.0627 | 37.5 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by 2-aminoheptanoic acid |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by cyclohexylalanine | 1.573 | 0.0583 | 27 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by cyclohexylalanine |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by D-isoleucine | 0.428 | 0.0788 | 5.5 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by D-isoleucine |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by L-leucine | 1.352 | 0.0403 | 33.5 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by L-leucine |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by L-valine | 0.973 | 0.0147 | 66.2 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by L-valine |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by norleucine | 0.93 | 0.0273 | 34 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by norleucine |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by norvaline | 2.43 | 0.0454 | 53.7 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 (TLKYPIEHGIVTNWDDMEKIWHH) with position 71 replaced by norvaline |
| SETD3 (histidine N3-methyltransferase) | Homo sapiens | β-actin peptide residues 66-88 TLKYPIEHGIVTNWDDMEKIWHH | 3.67 | 0.044 | 83.3 | pH 9, 37 °C | PMID 35142326 | Open record: SETD3 (histidine N3-methyltransferase), β-actin peptide residues 66-88 TLKYPIEHGIVTNWDDMEKIWHH |