EC 2.3.3.14: homocitrate synthase
TransferasesOpen in Browse
CatLog read 42 records for EC 2.3.3.14 from 4 papers, for 3 organisms. Names in these papers: homocitrate synthase (SpHCS), homocitrate synthase (StHCS), homocitrate synthase.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 26 records for EC 2.3.3.14 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.3.3.14 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Crystal structure and functional analysis of homocitrate synthase, an essential enzyme in lysine biosynthesis | Schizosaccharomyces pombe | 18 |
| Involvement of subdomain II in the recognition of acetyl-CoA revealed by the crystal structure of homocitrate synthase from Sulfolobus acidocaldarius | Sulfurisphaera tokodaii | 12 |
| Structural basis for L-lysine feedback inhibition of homocitrate synthase | Schizosaccharomyces pombe | 8 |
| Characterization of bacterial homocitrate synthase involved in lysine biosynthesis | Thermus thermophilus | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| homocitrate synthase | Thermus thermophilus | 2-oxoglutarate | 0.98 | 0.044 | 22 | pH 7.5, 50 °C | PMID 12095615 | Open record: homocitrate synthase, 2-oxoglutarate |
| homocitrate synthase | Thermus thermophilus | acetyl-CoA5 mM MgSO4; acetyl-CoA + 2-oxoglutarate | 1.53 | 0.032 | 48 | pH 7.5, 50 °C | PMID 12095615 | Open record: homocitrate synthase, acetyl-CoA |
| homocitrate synthase | Thermus thermophilus | acetyl-CoA10 mM KCl; cosubstrate oxaloacetate (saturating) | 0.97 | 0.028 | 35 | pH 7.5, 50 °C | PMID 12095615 | Open record: homocitrate synthase, acetyl-CoA |
| homocitrate synthase | Thermus thermophilus | oxaloacetate | 0.97 | 0.255 | 3.8 | pH 7.5, 50 °C | PMID 12095615 | Open record: homocitrate synthase, oxaloacetate |
| homocitrate synthase (SpHCS) | Schizosaccharomyces pombe | 2-oxoglutarate | 5.13 | 0.159 | 32.3 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS), 2-oxoglutarate |
| homocitrate synthase (SpHCS) | Schizosaccharomyces pombe | acetyl-CoA | 4.98 | 0.0107 | 467 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS), acetyl-CoA |
| homocitrate synthase (SpHCS)variant D123N | Schizosaccharomyces pombe | 2-oxoglutarate | 3.62 | 0.284 | 12.85 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant D123N, 2-oxoglutarate | |
| homocitrate synthase (SpHCS)variant D123N | Schizosaccharomyces pombe | acetyl coenzyme A | 3.62 | 0.0132 | 273 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant D123N, acetyl coenzyme A | |
| homocitrate synthase (SpHCS)variant E222Q | Schizosaccharomyces pombe | 2-oxoglutarate | 3.18 | 9.66 | 0.328 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant E222Q, 2-oxoglutarate | |
| homocitrate synthase (SpHCS)variant E222Q | Schizosaccharomyces pombe | acetyl coenzyme A | 3.58 | 0.0477 | 75.2 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant E222Q, acetyl coenzyme A | |
| homocitrate synthase (SpHCS)variant E74Q | Schizosaccharomyces pombe | 2-oxoglutarate | 0.778 | 0.791 | 0.983 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant E74Q, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant E74Q | Schizosaccharomyces pombe | acetyl-CoA | 0.742 | 0.0307 | 24.2 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant E74Q, acetyl-CoA |
| homocitrate synthase (SpHCS)variant H103A | Schizosaccharomyces pombe | 2-oxoglutarate | 0.303 | 3.56 | 0.0852 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant H103A, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant H103A | Schizosaccharomyces pombe | acetyl-CoA | 0.333 | 0.0352 | 9.47 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant H103A, acetyl-CoA |
| homocitrate synthase (SpHCS)variant Q364R | Schizosaccharomyces pombe | 2-oxoglutarate | 2.75 | 0.296 | 9.28 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant Q364R, 2-oxoglutarate | |
| homocitrate synthase (SpHCS)variant Q364R | Schizosaccharomyces pombe | acetyl coenzyme A | 2.83 | 0.0153 | 185 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant Q364R, acetyl coenzyme A | |
| homocitrate synthase (SpHCS)variant Q47A | Schizosaccharomyces pombe | 2-oxoglutarate | 0.558 | 4.94 | 0.113 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant Q47A, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant Q47A | Schizosaccharomyces pombe | acetyl-CoA | 0.583 | 0.0576 | 10.13 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant Q47A, acetyl-CoA |
| homocitrate synthase (SpHCS)variant R163K | Schizosaccharomyces pombe | 2-oxoglutarate | 0.435 | 24.4 | 0.0178 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant R163K, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant R163K | Schizosaccharomyces pombe | acetyl-CoA | 0.565 | 0.146 | 3.87 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant R163K, acetyl-CoA |
| homocitrate synthase (SpHCS)variant R288K | Schizosaccharomyces pombe | 2-oxoglutarate | 3.33 | 0.247 | 13.5 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant R288K, 2-oxoglutarate | |
| homocitrate synthase (SpHCS)variant R288K | Schizosaccharomyces pombe | acetyl coenzyme A | 3.23 | 0.014 | 232 | PMID 20089861 | Open record: homocitrate synthase (SpHCS) variant R288K, acetyl coenzyme A | |
| homocitrate synthase (SpHCS)variant S165A | Schizosaccharomyces pombe | 2-oxoglutarate | 2.67 | 0.536 | 4.97 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant S165A, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant S165A | Schizosaccharomyces pombe | acetyl-CoA | 2.63 | 0.0186 | 142 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant S165A, acetyl-CoA |
| homocitrate synthase (SpHCS)variant T197A | Schizosaccharomyces pombe | 2-oxoglutarate | 0.198 | 0.341 | 0.582 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant T197A, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant T197A | Schizosaccharomyces pombe | acetyl-CoA | 0.178 | 0.00744 | 24 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant T197A, acetyl-CoA |
| homocitrate synthase (SpHCS)variant T197S | Schizosaccharomyces pombe | 2-oxoglutarate | 2.28 | 0.229 | 9.95 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant T197S, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant T197S | Schizosaccharomyces pombe | acetyl-CoA | 2.07 | 0.006 | 343 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant T197S, acetyl-CoA |
| homocitrate synthase (SpHCS)variant Y332F | Schizosaccharomyces pombe | 2-oxoglutarate | 0.345 | 0.461 | 0.748 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant Y332F, 2-oxoglutarate |
| homocitrate synthase (SpHCS)variant Y332F | Schizosaccharomyces pombe | acetyl-CoA | 0.392 | 0.04 | 9.8 | pH 7.5 | PMID 19776021 | Open record: homocitrate synthase (SpHCS) variant Y332F, acetyl-CoA |
| homocitrate synthase (StHCS)variant G323N | Sulfurisphaera tokodaii | acetyl coenzyme A | 0.073 | 0.13 | 0.54 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant G323N, acetyl coenzyme A |
| homocitrate synthase (StHCS)variant G323N | Sulfurisphaera tokodaii | α-ketoglutarate | 0.17 | 0.039 | 4.3 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant G323N, α-ketoglutarate |
| homocitrate synthase (StHCS)variant H325A | Sulfurisphaera tokodaii | acetyl coenzyme A | 0.34 | 0.008 | 42 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant H325A, acetyl coenzyme A |
| homocitrate synthase (StHCS)variant H325A | Sulfurisphaera tokodaii | α-ketoglutarate | 0.26 | 0.0047 | 56 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant H325A, α-ketoglutarate |
| homocitrate synthase (StHCS)variant K324A | Sulfurisphaera tokodaii | acetyl coenzyme A | 0.55 | 0.014 | 40 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant K324A, acetyl coenzyme A |
| homocitrate synthase (StHCS)variant K324A | Sulfurisphaera tokodaii | α-ketoglutarate | 0.39 | 0.0079 | 49 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant K324A, α-ketoglutarate |
| homocitrate synthase (StHCS)variant K324A/H325A | Sulfurisphaera tokodaii | acetyl coenzyme A | 0.5 | 0.023 | 22 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant K324A/H325A, acetyl coenzyme A |
| homocitrate synthase (StHCS)variant K324A/H325A | Sulfurisphaera tokodaii | α-ketoglutarate | 0.25 | 0.0098 | 25 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant K324A/H325A, α-ketoglutarate |
| homocitrate synthase (StHCS)variant K350Y | Sulfurisphaera tokodaii | acetyl coenzyme A | 0.11 | 0.0036 | 30 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant K350Y, acetyl coenzyme A |
| homocitrate synthase (StHCS)variant K350Y | Sulfurisphaera tokodaii | α-ketoglutarate | 0.073 | 0.01 | 7.3 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant K350Y, α-ketoglutarate |
| homocitrate synthase (StHCS)variant R77A | Sulfurisphaera tokodaii | acetyl coenzyme A | 0.13 | 0.11 | 1.2 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant R77A, acetyl coenzyme A |
| homocitrate synthase (StHCS)variant R77A | Sulfurisphaera tokodaii | α-ketoglutarate | 0.17 | 0.047 | 3.6 | pH 7.5, 65 °C | PMID 32897601 | Open record: homocitrate synthase (StHCS) variant R77A, α-ketoglutarate |