EC 2.3.3.17: methylthioalkylmalate synthase
TransferasesOpen in Browse
CatLog read 22 records for EC 2.3.3.17 from 2 papers, for 2 organisms. Names in these papers: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 8 records for EC 2.3.3.17 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.3.3.17 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Kinetic and catalytic mechanisms of the methionine-derived glucosinolate biosynthesis enzyme methylthioalkylmalate synthase | Brassica juncea | 14 |
| MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis | Arabidopsis thaliana | 8 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | 3-methyl-2-oxobutanoate | 0.18 | 1 | 0.2 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), 3-methyl-2-oxobutanoate |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | 4-methylthio-2-oxobutanoic acid | 1.3 | 0.932 | 1.38 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | 5-methylthio-2-oxopentanoic acid | 1.3 | 0.476 | 2.73 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), 5-methylthio-2-oxopentanoic acid |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | 6-methylthio-2-oxohexanoic acid | 2.5 | 0.463 | 5.4 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), 6-methylthio-2-oxohexanoic acid |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | 8-methylthio-2-oxooctanoic acid | 0.3 | 0.253 | 1.28 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), 8-methylthio-2-oxooctanoic acid |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | 9-methylthio-2-oxononanoic acid | 0.03 | 0.081 | 0.37 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), 9-methylthio-2-oxononanoic acid |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | acetyl-coenzyme A | 3 | 2.3 | 1.3 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), acetyl-coenzyme A |
| methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L) | Arabidopsis thaliana | pyruvate | 0.17 | 8.6 | 0.023 | pH 8, 32 °C | PMID 17369439 | Open record: methylthioalkylmalate synthase 3 (MAM3, formerly MAM-L), pyruvate |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | 4-methylthio-2-oxobutanoic acidTable 1 | 0.1118 | 0.0246 | 2.796 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | 4-methylthio-2-oxobutanoic acidMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.1162 | 0.0311 | 3.735 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | 4-methylthio-2-oxobutanoic acidMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.1097 | 0.0511 | 2.146 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | acetyl-coenzyme ATable 1 | — | 0.04 | 4.546 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | acetyl-coenzyme AMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.133 | 0.0634 | 2.098 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | acetyl-coenzyme AMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.112 | 0.0246 | 4.553 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant E227Q | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0092 | 0.053 | 0.173 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant E227Q, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant E227Q | Brassica juncea | acetyl-coenzyme A | 0.0097 | 0.174 | 0.056 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant E227Q, acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant H388N | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0033 | 0.0379 | 0.088 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant H388N, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant H388N | Brassica juncea | acetyl-coenzyme A | 0.0052 | 0.0704 | 0.073 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant H388N, acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant T257S | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0853 | 0.169 | 0.505 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant T257S, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant T257S | Brassica juncea | acetyl-coenzyme A | 0.0997 | 0.238 | 0.419 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant T257S, acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant Y399F | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0027 | 0.0996 | 0.027 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant Y399F, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant Y399F | Brassica juncea | acetyl-coenzyme A | 0.0028 | 0.14 | 0.02 | pH 7.5, 25 °C | PMID 39322014 | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant Y399F, acetyl-coenzyme A |