Values CatLog read from this paper
Kinetic and catalytic mechanisms of the methionine-derived glucosinolate biosynthesis enzyme methylthioalkylmalate synthase
PMID 39322014doi:10.1016/j.jbc.2024.107814Open in Browse
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CatLog read 14 records (41 values) from this paper: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) from Brassica juncea.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | 4-methylthio-2-oxobutanoic acidTable 1 | 0.1118 | 0.0246 | 2.796 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | 4-methylthio-2-oxobutanoic acidMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.1162 | 0.0311 | 3.735 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | 4-methylthio-2-oxobutanoic acidMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.1097 | 0.0511 | 2.146 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | acetyl-coenzyme ATable 1 | — | 0.04 | 4.546 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | acetyl-coenzyme AMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.133 | 0.0634 | 2.098 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A) | Brassica juncea | acetyl-coenzyme AMichaelis-Menten fit (GraphPad Prism); Table 2 | 0.112 | 0.0246 | 4.553 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A), acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant E227Q | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0092 | 0.053 | 0.173 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant E227Q, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant E227Q | Brassica juncea | acetyl-coenzyme A | 0.0097 | 0.174 | 0.056 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant E227Q, acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant H388N | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0033 | 0.0379 | 0.088 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant H388N, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant H388N | Brassica juncea | acetyl-coenzyme A | 0.0052 | 0.0704 | 0.073 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant H388N, acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant T257S | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0853 | 0.169 | 0.505 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant T257S, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant T257S | Brassica juncea | acetyl-coenzyme A | 0.0997 | 0.238 | 0.419 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant T257S, acetyl-coenzyme A |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant Y399F | Brassica juncea | 4-methylthio-2-oxobutanoic acid | 0.0027 | 0.0996 | 0.027 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant Y399F, 4-methylthio-2-oxobutanoic acid |
| methylthioalkylmalate synthase MAMS2A (BjMAMS2A)variant Y399F | Brassica juncea | acetyl-coenzyme A | 0.0028 | 0.14 | 0.02 | pH 7.5, 25 °C | Open record: methylthioalkylmalate synthase MAMS2A (BjMAMS2A) variant Y399F, acetyl-coenzyme A |