CatLogenzyme kinetics catalog

EC 2.4.1.4: amylosucrase

TransferasesOpen in Browse

CatLog read 19 records for EC 2.4.1.4 from 4 papers, for 2 organisms. Names in these papers: amylosucrase (NpAS), amylosucrase (BtAS), amylosucrase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 25 records for EC 2.4.1.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.1.4 in Browse.

Papers

PaperOrganismsRecords
Essential role of amino acid position 226 in oligosaccharide elongation by amylosucrase from Neisseria polysacchareaNeisseria polysaccharea10
Modulation of Fructose Transfer Process for Promoting Apparent Isomerization Activity of Amylosucrase from Bifidobacterium thermophilumBifidobacterium thermophilum5
Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucraseNeisseria polysaccharea3
Engineering of anp efficient mutant of Neisseria polysaccharea amylosucrase for the synthesis of controlled size maltooligosaccharidesNeisseria polysaccharea1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
amylosucrasevariant R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437R/N439DNeisseria polysacchareasucrose——0.01230 °CPMID 28019698Open
amylosucrasevariant R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445RNeisseria polysacchareasucrose4.578.430.05830 °CPMID 28019698Open
amylosucrasevariant R226K/I228V/A289I/F290Y/E300I/V331T/Q437S/N439D/C445ANeisseria polysacchareasucrose1.61128.30.01330 °CPMID 28732882Open
amylosucrasevariant R226L/I228V/F229A/A289I/F290Y/E300I/V331TNeisseria polysacchareasucrose——0.01530 °CPMID 28019698Open
amylosucrase (BtAS)Bifidobacterium thermophilumfructoseturanose quantified by HPAEC-PAD—77.06—pH 6, 50 °CPMID 41937541Open
amylosucrase (BtAS)Bifidobacterium thermophilumfructosetrehalulose quantified by HPAEC-PAD—10.78—pH 6, 50 °CPMID 41937541Open
amylosucrase (BtAS)variant G374HBifidobacterium thermophilumfructose—56.2—pH 6, 50 °CPMID 41937541Open
amylosucrase (BtAS)variant G374TBifidobacterium thermophilumfructoseturanose quantified by HPAEC-PAD—36.03—pH 6, 50 °CPMID 41937541Open
amylosucrase (BtAS)variant G374TBifidobacterium thermophilumfructosetrehalulose quantified by HPAEC-PAD—34.02—pH 6, 50 °CPMID 41937541Open
amylosucrase (NpAS)Neisseria polysacchareaglucose3.1110.28pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)Neisseria polysacchareamaltose4.8160.3pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)Neisseria polysacchareamaltotetraose47610.77pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)Neisseria polysacchareamaltotriose7.5300.25pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)Neisseria polysacchareasucrose0.482.50.19pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)variant R226NNeisseria polysacchareaglucose14140.99pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)variant R226NNeisseria polysacchareamaltose3312.32.7pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)variant R226NNeisseria polysacchareamaltotetraose173247.2pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)variant R226NNeisseria polysacchareamaltotriose67262.6pH 7, 30 °CPMID 24801911Open
amylosucrase (NpAS)variant R226NNeisseria polysacchareasucrose1.48.10.17pH 7, 30 °CPMID 24801911Open