CatLogenzyme kinetics catalog

EC 2.4.2.12: nicotinamide phosphoribosyltransferase

TransferasesOpen in Browse

CatLog read 13 records for EC 2.4.2.12 from 2 papers, for 2 organisms. Names in these papers: nicotinamide phosphoribosyltransferase (NAMPT), nicotinamide phosphoribosyltransferase (NadV).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 20 records for EC 2.4.2.12 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.2.12 in Browse.

Papers

PaperOrganismsRecords
Weak coupling of ATP hydrolysis to the chemical equilibrium of human nicotinamide phosphoribosyltransferaseHomo sapiens11
Genomics-driven reconstruction of acinetobacter NAD metabolism: insights for antibacterial target selectionAcinetobacter baylyi2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
nicotinamide phosphoribosyltransferase (NadV)Acinetobacter baylyinicotinamide0.120.043pH 7.5, 30 °CPMID 20926389Open
nicotinamide phosphoribosyltransferase (NadV)Acinetobacter baylyinicotinic acid0.02306.7 × 10⁻⁴pH 7.5, 30 °CPMID 20926389Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensadenosine 5'-diphosphate0.0150.440.034 calc.pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensadenosine 5'-triphosphateATP-coupled NMN synthesis; 5 mM MgCl2—7.4—pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensadenosine 5'-triphosphateADP and ATP as equimolar Mg complexes; 5 mM free MgCl2—2.2—pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensalpha-D-5-phosphoribosyl-1-pyrophosphatenon ATP-coupled NMN synthesis; no Pi; 88 nM enzyme—0.0072—pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensalpha-D-5-phosphoribosyl-1-pyrophosphate88 nM enzyme; cosubstrate NAM (1 µM)—0.0117—pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensalpha-D-5-phosphoribosyl-1-pyrophosphateATP-coupled NMN synthesis—6.3 × 10⁻⁴—pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensnicotinamidenon ATP-coupled NMN synthesis; no Pi; 50 mM HEPES pH 7.50.001378.55 × 10⁻⁴1.6pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensnicotinamideradiolabeled [14C]NAM assay with HPLC (Method 1); 50 mM NaCl0.001422.35 × 10⁻⁴6.1pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensnicotinamideATP-coupled NMN synthesis; 50 mM HEPES pH 7.5; 50 mM NaCl0.00775 × 10⁻⁶1.8 × 10³pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensnicotinamideATP-coupled NMN synthesis; continuous fluorometric assay—5.7 × 10⁻⁵—pH 7.5, 25 °CPMID 18823127Open
nicotinamide phosphoribosyltransferase (NAMPT)Homo sapiensnicotinamideradiolabeled [14C]NAM assay with HPLC (Method 1); 50 mM NaCl—1.7 × 10⁻⁵—pH 7.5, 25 °CPMID 18823127Open