Values CatLog read from this paper
Weak coupling of ATP hydrolysis to the chemical equilibrium of human nicotinamide phosphoribosyltransferase
PMID 18823127doi:10.1021/bi801198mOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (18 values) from this paper: nicotinamide phosphoribosyltransferase (NAMPT) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | adenosine 5'-diphosphate | 0.015 | 0.44 | 0.034 calc. | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), adenosine 5'-diphosphate |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | adenosine 5'-triphosphateATP-coupled NMN synthesis; 5 mM MgCl2 | — | 7.4 | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), adenosine 5'-triphosphate |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | adenosine 5'-triphosphateADP and ATP as equimolar Mg complexes; 5 mM free MgCl2 | — | 2.2 | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), adenosine 5'-triphosphate |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | alpha-D-5-phosphoribosyl-1-pyrophosphatenon ATP-coupled NMN synthesis; no Pi; 88 nM enzyme | — | 0.0072 | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), alpha-D-5-phosphoribosyl-1-pyrophosphate |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | alpha-D-5-phosphoribosyl-1-pyrophosphate88 nM enzyme; cosubstrate NAM (1 µM) | — | 0.0117 | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), alpha-D-5-phosphoribosyl-1-pyrophosphate |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | alpha-D-5-phosphoribosyl-1-pyrophosphateATP-coupled NMN synthesis | — | 6.3 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), alpha-D-5-phosphoribosyl-1-pyrophosphate |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | nicotinamidenon ATP-coupled NMN synthesis; no Pi; 50 mM HEPES pH 7.5 | 0.00137 | 8.55 × 10⁻⁴ | 1.6 | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), nicotinamide |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | nicotinamideradiolabeled [14C]NAM assay with HPLC (Method 1); 50 mM NaCl | 0.00142 | 2.35 × 10⁻⁴ | 6.1 | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), nicotinamide |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | nicotinamideATP-coupled NMN synthesis; 50 mM HEPES pH 7.5; 50 mM NaCl | 0.0077 | 5 × 10⁻⁶ | 1.8 × 10³ | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), nicotinamide |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | nicotinamideATP-coupled NMN synthesis; continuous fluorometric assay | — | 5.7 × 10⁻⁵ | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), nicotinamide |
| nicotinamide phosphoribosyltransferase (NAMPT) | Homo sapiens | nicotinamideradiolabeled [14C]NAM assay with HPLC (Method 1); 50 mM NaCl | — | 1.7 × 10⁻⁵ | — | pH 7.5, 25 °C | Open record: nicotinamide phosphoribosyltransferase (NAMPT), nicotinamide |