CatLogenzyme kinetics catalog

EC 2.4.2.14: amidophosphoribosyltransferase

TransferasesOpen in Browse

CatLog read 40 records for EC 2.4.2.14 from 1 paper, for 1 organism. Names in these papers: glutamine phosphoribosylpyrophosphate amidotransferase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 20 records for EC 2.4.2.14 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.2.14 in Browse.

Papers

PaperOrganismsRecords
Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate siteEscherichia coli40

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coli5-phospho-alpha-D-ribose 1-diphosphate—0.053—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliammonia—7.34—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—3.24—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—1.72—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc; Table I—2.1—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted); Table I—193—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)——0.047pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA——14.98pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—2.64—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseAla insert (Ala inserted between Met-1 and Cys1)Escherichia coliammonia—8.49—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferaseAla insert (Ala inserted between Met-1 and Cys1)Escherichia coliL-glutamine—1.48—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant D127AEscherichia coliammonia—6.08—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant D127AEscherichia coliL-glutamine—236—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant G102AEscherichia coliammonia—7.67—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant G102AEscherichia coliL-glutamine—2.43—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101DEscherichia coliammonia—9.17—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101DEscherichia coliL-glutamine—1.42—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101GEscherichia coliammonia—6.03—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101GEscherichia coliL-glutamine—0.64—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73HEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—101—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73HEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—111—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73HEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc; Table V—153—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—110—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—115—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73LEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc; Table V—161—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73L/Y74LEscherichia coliammonia—34.1—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73L/Y74LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; cosubstrate PRPP—185—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73L/Y74LEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—185—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coli5-phospho-alpha-D-ribose 1-diphosphate—0.04—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliammonia—12—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—30—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc—32.8—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—240—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliammonia—21.5—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—46—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc—13.5—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—179—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74SEscherichia coliammonia—30.7—pH 8.5, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74SEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; cosubstrate PRPP—228—pH 8, 37 °CPMID 8663035Open
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74SEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—228—pH 8, 37 °CPMID 8663035Open