CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site

PMID 8663035doi:10.1074/jbc.271.26.15549Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (40 values) from this paper: glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.2.14

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coli5-phospho-alpha-D-ribose 1-diphosphate—0.053—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliammonia—7.34—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—3.24—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—1.72—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc; Table I—2.1—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted); Table I—193—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)——0.047pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA——14.98pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—2.64—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseAla insert (Ala inserted between Met-1 and Cys1)Escherichia coliammonia—8.49—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferaseAla insert (Ala inserted between Met-1 and Cys1)Escherichia coliL-glutamine—1.48—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant D127AEscherichia coliammonia—6.08—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant D127AEscherichia coliL-glutamine—236—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant G102AEscherichia coliammonia—7.67—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant G102AEscherichia coliL-glutamine—2.43—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101DEscherichia coliammonia—9.17—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101DEscherichia coliL-glutamine—1.42—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101GEscherichia coliammonia—6.03—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant N101GEscherichia coliL-glutamine—0.64—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73HEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—101—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73HEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—111—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73HEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc; Table V—153—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—110—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—115—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73LEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc; Table V—161—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73L/Y74LEscherichia coliammonia—34.1—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73L/Y74LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; cosubstrate PRPP—185—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant R73L/Y74LEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—185—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coli5-phospho-alpha-D-ribose 1-diphosphate—0.04—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliammonia—12—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—30—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc—32.8—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74FEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—240—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliammonia—21.5—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; 1 mg/ml BSA—46—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliL-glutamineglutamine-dependent PRA synthesis; 10 mM MgOAc—13.5—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74LEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—179—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74SEscherichia coliammonia—30.7—pH 8.5, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74SEscherichia coliL-glutamineglutamine varied: 2.5 mM PRPP; 10 mM MgCl2; cosubstrate PRPP—228—pH 8, 37 °COpen
glutamine phosphoribosylpyrophosphate amidotransferasevariant Y74SEscherichia coliL-glutaminebasal PRPP-independent glutaminase (PRPP omitted)—228—pH 8, 37 °COpen