CatLogenzyme kinetics catalog

EC 2.4.99.28: peptidoglycan glycosyltransferase

TransferasesOpen in Browse

CatLog read 20 records for EC 2.4.99.28 from 2 papers, for 1 organism. Names in these papers: PBP1B, penicillin-binding protein 1b (PBP1b).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 3 records for EC 2.4.99.28 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.99.28 in Browse.

Papers

PaperOrganismsRecords
Substrate and Stereochemical Control of Peptidoglycan Cross-Linking by Transpeptidation by Escherichia coli PBP1BEscherichia coli14
Importance of the conserved residues in the peptidoglycan glycosyltransferase module of the class A penicillin-binding protein 1b of Escherichia coliEscherichia coli6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
PBP1BEscherichia coliD-cycloserine0.09650.06431.5pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliD-lactate0.41850.085pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliL-alanine1.131700.0067pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia colilipid II-Lys0.04530.00875.2 calc.pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc L-alanyl-gamma-D-glutamyl-meso-DAP20 µM lipid II-Lys donor added before PBP1B; 11.0 nM PBP1B1.270.03537pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc L-alanyl-gamma-D-glutamyl-meso-DAP9.6 nM PBP1B; global fit of the full matrix1.4——pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanine0.810.0081100pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanyl-D-alanine1.830.04145pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc meso-DAP pentapeptide-NH21.450.02460pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc meso-DAP tetrapeptide-NH23.20.04667pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliMurNAc meso-DAP tripeptide-NH21.80.02962pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliundecaprenyl pyrophosphoryl GlcNAc-MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanine0.680.009770pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliundecaprenyl pyrophosphoryl GlcNAc-MurNAc L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine2.7——pH 8.5, 30 °CPMID 32048840Open
PBP1BEscherichia coliundecaprenyl pyrophosphoryl MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanyl-D-alanine0.70.01937pH 8.5, 30 °CPMID 32048840Open
penicillin-binding protein 1b (PBP1b)variant G242AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.00290.00161.8pH 7.5, 30 °CPMID 18701463Open
penicillin-binding protein 1b (PBP1b)variant H240QEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.00520.00184.7pH 7.5, 30 °CPMID 18701463Open
penicillin-binding protein 1b (PBP1b)variant K287AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.0440.001236.5pH 7.5, 30 °CPMID 18701463Open
penicillin-binding protein 1b (PBP1b)variant N312AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.0110.00129.2pH 7.5, 30 °CPMID 18701463Open
penicillin-binding protein 1b (PBP1b)variant R372AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.01340.001112.2pH 7.5, 30 °CPMID 18701463Open
penicillin-binding protein 1b (PBP1b)variant S266AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.00760.00126.3pH 7.5, 30 °CPMID 18701463Open