Values CatLog read from this paper

Importance of the conserved residues in the peptidoglycan glycosyltransferase module of the class A penicillin-binding protein 1b of Escherichia coli

PMID 18701463doi:10.1074/jbc.m803223200Open in Browse

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CatLog read 6 records (18 values) from this paper: penicillin-binding protein 1b (PBP1b) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.99.28, EC 3.4.16.4

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
penicillin-binding protein 1b (PBP1b)variant G242AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.00290.00161.8pH 7.5, 30 °COpen record: penicillin-binding protein 1b (PBP1b) variant G242A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant H240QEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.00520.00184.7pH 7.5, 30 °COpen record: penicillin-binding protein 1b (PBP1b) variant H240Q, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant K287AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.0440.001236.5pH 7.5, 30 °COpen record: penicillin-binding protein 1b (PBP1b) variant K287A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant N312AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.0110.00129.2pH 7.5, 30 °COpen record: penicillin-binding protein 1b (PBP1b) variant N312A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant R372AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.01340.001112.2pH 7.5, 30 °COpen record: penicillin-binding protein 1b (PBP1b) variant R372A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant S266AEscherichia coliN-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol0.00760.00126.3pH 7.5, 30 °COpen record: penicillin-binding protein 1b (PBP1b) variant S266A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol