EC 3.4.16.4: serine-type D-Ala-D-Ala carboxypeptidase

HydrolasesOpen in Browse

CatLog read 65 records for EC 3.4.16.4 from 7 papers, for 7 organisms. Names in these papers: Streptomyces K15 DD-transpeptidase/penicillin-binding protein, PBP1B, PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), penicillin-binding protein 1b (PBP1b) and 5 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 27 records for EC 3.4.16.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.16.4 in Browse.

Papers

Papers with records for EC 3.4.16.4
Paper	Organisms	Records
Catalytic mechanism of the Streptomyces K15 DD-transpeptidase/penicillin-binding protein probed by site-directed mutagenesis and structural analysis	Streptomyces sp. K15	31
Substrate and Stereochemical Control of Peptidoglycan Cross-Linking by Transpeptidation by Escherichia coli PBP1B	Escherichia coli	14
Purification and characterization of PBP4a, a new low-molecular-weight penicillin-binding protein from Bacillus subtilis	Actinomadura sp., Bacillus subtilis	7
Importance of the conserved residues in the peptidoglycan glycosyltransferase module of the class A penicillin-binding protein 1b of Escherichia coli	Escherichia coli	6
Catalytic mechanism of active-site serine beta-lactamases: role of the conserved hydroxy group of the Lys-Thr(Ser)-Gly triad	Escherichia coli	4
Kinetics and stereochemistry of hydrolysis of an N-(phenylacetyl)-α-hydroxyglycine ester catalyzed by serine β-lactamases and DD-peptidases	Actinomadura sp. R39, Streptomyces sp. R61	2
Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis	Ochrobactrum anthropi	1

Records

Records for EC 3.4.16.4 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
D-aminopeptidase (DAP)variant 475G487	Ochrobactrum anthropi	Na,Na-Ac2-L-Lys-D-Ala-D-Ala	0.09	200	4.5 × 10⁻⁴	pH 8, 37 °C	PMID 16131658	Open record: D-aminopeptidase (DAP) variant 475G487, Na,Na-Ac2-L-Lys-D-Ala-D-Ala
PBP1B	Escherichia coli	D-cycloserine	0.0965	0.0643	1.5	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, D-cycloserine
PBP1B	Escherichia coli	D-lactate	0.418	5	0.085	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, D-lactate
PBP1B	Escherichia coli	L-alanine	1.13	170	0.0067	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, L-alanine
PBP1B	Escherichia coli	lipid II-Lys	0.0453	0.0087	5.2 calc.	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, lipid II-Lys
PBP1B	Escherichia coli	MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP20 µM lipid II-Lys donor added before PBP1B; 11.0 nM PBP1B	1.27	0.035	37	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP
PBP1B	Escherichia coli	MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP9.6 nM PBP1B; global fit of the full matrix	1.4	—	—	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP
PBP1B	Escherichia coli	MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanine	0.81	0.0081	100	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanine
PBP1B	Escherichia coli	MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanyl-D-alanine	1.83	0.041	45	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanyl-D-alanine
PBP1B	Escherichia coli	MurNAc meso-DAP pentapeptide-NH2	1.45	0.024	60	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc meso-DAP pentapeptide-NH2
PBP1B	Escherichia coli	MurNAc meso-DAP tetrapeptide-NH2	3.2	0.046	67	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc meso-DAP tetrapeptide-NH2
PBP1B	Escherichia coli	MurNAc meso-DAP tripeptide-NH2	1.8	0.029	62	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, MurNAc meso-DAP tripeptide-NH2
PBP1B	Escherichia coli	undecaprenyl pyrophosphoryl GlcNAc-MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanine	0.68	0.0097	70	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, undecaprenyl pyrophosphoryl GlcNAc-MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanine
PBP1B	Escherichia coli	undecaprenyl pyrophosphoryl GlcNAc-MurNAc L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine	2.7	—	—	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, undecaprenyl pyrophosphoryl GlcNAc-MurNAc L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
PBP1B	Escherichia coli	undecaprenyl pyrophosphoryl MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanyl-D-alanine	0.7	0.019	37	pH 8.5, 30 °C	PMID 32048840	Open record: PBP1B, undecaprenyl pyrophosphoryl MurNAc L-alanyl-gamma-D-glutamyl-meso-DAP-D-alanyl-D-alanine
PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase)	Bacillus subtilis	benzoyl-D-Ala-thioglycolate	23	0.38	61	pH 7.5, 30 °C	PMID 11160090	Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-D-Ala-thioglycolate
PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase)	Bacillus subtilis	benzoyl-D-Ala-thioglycolate10 mM D-alanine; cosubstrate D-alanine (10 mM)	28	0.43	65	pH 7.5, 30 °C	PMID 11160090	Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-D-Ala-thioglycolate
PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase)	Bacillus subtilis	benzoyl-D-Ala-thioglycolate50 mM D-alanine; cosubstrate D-alanine (50 mM)	42	0.54	78	pH 7.5, 30 °C	PMID 11160090	Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-D-Ala-thioglycolate
PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase)	Bacillus subtilis	benzoyl-Gly-thioglycolate	2	0.47	4.3	pH 7.5, 30 °C	PMID 11160090	Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-Gly-thioglycolate
PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase)	Bacillus subtilis	Nalpha-acetyl-L-Lys-D-Ala-D-Ala	—	—	6.7	pH 7.5, 30 °C	PMID 11160090	Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), Nalpha-acetyl-L-Lys-D-Ala-D-Ala
PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase)	Bacillus subtilis	Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate	—	—	35	pH 7.5, 30 °C	PMID 11160090	Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate
penicillin-binding protein 1b (PBP1b)variant G242A	Escherichia coli	N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol	0.0029	0.0016	1.8	pH 7.5, 30 °C	PMID 18701463	Open record: penicillin-binding protein 1b (PBP1b) variant G242A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant H240Q	Escherichia coli	N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol	0.0052	0.0018	4.7	pH 7.5, 30 °C	PMID 18701463	Open record: penicillin-binding protein 1b (PBP1b) variant H240Q, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant K287A	Escherichia coli	N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol	0.044	0.0012	36.5	pH 7.5, 30 °C	PMID 18701463	Open record: penicillin-binding protein 1b (PBP1b) variant K287A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant N312A	Escherichia coli	N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol	0.011	0.0012	9.2	pH 7.5, 30 °C	PMID 18701463	Open record: penicillin-binding protein 1b (PBP1b) variant N312A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant R372A	Escherichia coli	N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol	0.0134	0.0011	12.2	pH 7.5, 30 °C	PMID 18701463	Open record: penicillin-binding protein 1b (PBP1b) variant R372A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
penicillin-binding protein 1b (PBP1b)variant S266A	Escherichia coli	N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol	0.0076	0.0012	6.3	pH 7.5, 30 °C	PMID 18701463	Open record: penicillin-binding protein 1b (PBP1b) variant S266A, N-acetylglucosaminyl-N-acetylmuramoyl(L-Ala-gamma-D-Glu-(L)-meso-A2pm-(L)-D-Ala-D-Ala)-pyrophosphate-undecaprenol
R39 DD-peptidase	Actinomadura sp.	Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate	5.4	0.042	130	pH 7.5, 30 °C	PMID 11160090	Open record: R39 DD-peptidase, Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate
R39 DD-peptidase (LMM class C)	Actinomadura sp. R39	3-carboxyphenyl N-(phenylacetyl)-α-hydroxyglycinate (D(R) enantiomer)	10.4	4.3	2.4	pH 7.5, 25 °C	PMID 22851054	Open record: R39 DD-peptidase (LMM class C), 3-carboxyphenyl N-(phenylacetyl)-α-hydroxyglycinate (D(R) enantiomer)
R61 DD-peptidase (LMM class B)	Streptomyces sp. R61	3-carboxyphenyl N-(phenylacetyl)-α-hydroxyglycinate (D(R) enantiomer)	6.9	0.36	19	pH 7.5, 25 °C	PMID 22851054	Open record: R61 DD-peptidase (LMM class B), 3-carboxyphenyl N-(phenylacetyl)-α-hydroxyglycinate (D(R) enantiomer)
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	benzoyl-D-alanyl-thioglycolate	0.3	1.3	0.2	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, benzoyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	benzoyl-glycyl-thioglycolate	0.08	0.8	0.1	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, benzoyl-glycyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	benzoyl-glycyl-thiolactate	0.08	0.3	0.27	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, benzoyl-glycyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine	—	—	0.006	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate	1.1	2	0.58	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine	0.13	30	0.0043	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate	0.8	0.6	1.3	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	phenylacetyl-D-alanyl-thioglycolate	—	—	0.29	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, phenylacetyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A	Streptomyces sp. K15	phenylacetyl-D-alanyl-thiolactate	2.2	1.2	2	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, phenylacetyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	benzoyl-D-alanyl-thioglycolate	—	—	0.26	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, benzoyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	benzoyl-glycyl-thioglycolate	—	—	0.085	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, benzoyl-glycyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	benzoyl-glycyl-thiolactate	0.45	3.3	0.14	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, benzoyl-glycyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine	—	—	0.001	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate	—	—	0.19	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine	—	—	7 × 10⁻⁴	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate	—	—	0.29	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	phenylacetyl-D-alanyl-thioglycolate	—	—	0.32	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, phenylacetyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N	Streptomyces sp. K15	phenylacetyl-D-alanyl-thiolactate	—	—	0.8	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, phenylacetyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H	Streptomyces sp. K15	benzoyl-D-alanyl-thioglycolate	—	—	0.005	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, benzoyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H	Streptomyces sp. K15	benzoyl-glycyl-thiolactate	0.006	1	0.006	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, benzoyl-glycyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H	Streptomyces sp. K15	phenylacetyl-D-alanyl-thioglycolate	0.015	2.4	0.0064	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, phenylacetyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H	Streptomyces sp. K15	phenylacetyl-D-alanyl-thiolactate	—	—	0.0011	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, phenylacetyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	benzoyl-D-alanyl-thioglycolate	—	—	0.004	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, benzoyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	benzoyl-glycyl-thioglycolate	—	—	0.0024	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, benzoyl-glycyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	benzoyl-glycyl-thiolactate	—	—	0.015	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, benzoyl-glycyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine	—	—	2.7 × 10⁻⁴	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate	—	—	0.0068	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine	—	—	1.5 × 10⁻⁴	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate	—	—	0.0076	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	phenylacetyl-D-alanyl-thioglycolate	—	—	0.008	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, phenylacetyl-D-alanyl-thioglycolate
Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A	Streptomyces sp. K15	phenylacetyl-D-alanyl-thiolactate	—	—	0.004	pH 7.2, 37 °C	PMID 12627955	Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, phenylacetyl-D-alanyl-thiolactate
TEM-1 beta-lactamase	Escherichia coli	carboxymethyl benzoylaminothioacetate	20	3.8	5.2	pH 7, 30 °C	PMID 8042993	Open record: TEM-1 beta-lactamase, carboxymethyl benzoylaminothioacetate
TEM-1 beta-lactamase	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	0.1	1.92	0.052	pH 7, 30 °C	PMID 8042993	Open record: TEM-1 beta-lactamase, DL-phenyl-lactyl benzoylaminoacetate
TEM-1 beta-lactamasevariant S235A	Escherichia coli	carboxymethyl benzoylaminothioacetate	—	—	0.3	pH 7, 30 °C	PMID 8042993	Open record: TEM-1 beta-lactamase variant S235A, carboxymethyl benzoylaminothioacetate
TEM-1 beta-lactamasevariant S235A	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	—	—	0.004	pH 7, 30 °C	PMID 8042993	Open record: TEM-1 beta-lactamase variant S235A, DL-phenyl-lactyl benzoylaminoacetate