Values CatLog read from this paper

Catalytic mechanism of active-site serine beta-lactamases: role of the conserved hydroxy group of the Lys-Thr(Ser)-Gly triad

PMID 8042993doi:10.1042/bj3010485Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 97 records (249 values) from this paper: AmpC beta-lactamase, TEM-1 beta-lactamase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.16.4, EC 3.5.2.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
AmpC beta-lactamase	Escherichia coli	(3-Hydroxymethyl)phenoxymethylpenicillin	11	0.00225	4.9 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase, (3-Hydroxymethyl)phenoxymethylpenicillin
AmpC beta-lactamase	Escherichia coli	Ampicillin	3	0.00307	980	pH 8, 30 °C	Open record: AmpC beta-lactamase, Ampicillin
AmpC beta-lactamase	Escherichia coli	Aztreonam	0.0024	—	410	pH 8, 30 °C	Open record: AmpC beta-lactamase, Aztreonam
AmpC beta-lactamase	Escherichia coli	Benzylpenicillin	86	0.0045	1.95 × 10⁴	pH 8, 30 °C	Open record: AmpC beta-lactamase, Benzylpenicillin
AmpC beta-lactamase	Escherichia coli	Benzylpenicillin methyl ester	43	0.00175	2.4 × 10⁴	pH 8, 30 °C	Open record: AmpC beta-lactamase, Benzylpenicillin methyl ester
AmpC beta-lactamase	Escherichia coli	Carbenicillin	0.0044	3 × 10⁻⁵	135	pH 8, 30 °C	Open record: AmpC beta-lactamase, Carbenicillin
AmpC beta-lactamase	Escherichia coli	Carbenicillin methyl ester	0.006	3 × 10⁻⁴	23	pH 8, 30 °C	Open record: AmpC beta-lactamase, Carbenicillin methyl ester
AmpC beta-lactamase	Escherichia coli	carboxymethyl benzoylaminothioacetate	—	—	2.9	pH 8, 30 °C	Open record: AmpC beta-lactamase, carboxymethyl benzoylaminothioacetate
AmpC beta-lactamase	Escherichia coli	Cefazolin	477	1.51	320	pH 8, 30 °C	Open record: AmpC beta-lactamase, Cefazolin
AmpC beta-lactamase	Escherichia coli	Cefotaxime	0.15	8.7 × 10⁻⁴	170	pH 8, 30 °C	Open record: AmpC beta-lactamase, Cefotaxime
AmpC beta-lactamase	Escherichia coli	Cefuroxime	0.05	8.7 × 10⁻⁵	575	pH 8, 30 °C	Open record: AmpC beta-lactamase, Cefuroxime
AmpC beta-lactamase	Escherichia coli	Cephaloridine	188	0.19	990	pH 8, 30 °C	Open record: AmpC beta-lactamase, Cephaloridine
AmpC beta-lactamase	Escherichia coli	Cephalosporin C	540	0.074	7.3 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase, Cephalosporin C
AmpC beta-lactamase	Escherichia coli	deacetylcephalosporin C	364	0.05	7.2 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase, deacetylcephalosporin C
AmpC beta-lactamase	Escherichia coli	Deacetylcephalosporin C lactone	113	0.0186	6.1 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase, Deacetylcephalosporin C lactone
AmpC beta-lactamase	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	30	1.32	22.7	pH 8, 30 °C	Open record: AmpC beta-lactamase, DL-phenyl-lactyl benzoylaminoacetate
AmpC beta-lactamase	Escherichia coli	Imipenem	0.005	—	9.3	pH 8, 30 °C	Open record: AmpC beta-lactamase, Imipenem
AmpC beta-lactamase	Escherichia coli	Nitrocefin	670	0.52	1.29 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase, Nitrocefin
AmpC beta-lactamase	Escherichia coli	Phenoxymethylpenicillin	43	0.0069	6.3 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase, Phenoxymethylpenicillin
AmpC beta-lactamasevariant T316A	Escherichia coli	(3-Hydroxymethyl)phenoxymethylpenicillin	4.8	0.0029	2.3 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, (3-Hydroxymethyl)phenoxymethylpenicillin
AmpC beta-lactamasevariant T316A	Escherichia coli	Ampicillin	2.5	0.00373	670	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Ampicillin
AmpC beta-lactamasevariant T316A	Escherichia coli	Aztreonam	0.0014	—	77	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Aztreonam
AmpC beta-lactamasevariant T316A	Escherichia coli	Benzylpenicillin	83	0.0122	6.8 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Benzylpenicillin
AmpC beta-lactamasevariant T316A	Escherichia coli	Benzylpenicillin methyl ester	8.4	3 × 10⁻⁴	2.8 × 10⁴	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Benzylpenicillin methyl ester
AmpC beta-lactamasevariant T316A	Escherichia coli	Carbenicillin	0.0031	4 × 10⁻⁴	7.4	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Carbenicillin
AmpC beta-lactamasevariant T316A	Escherichia coli	Carbenicillin methyl ester	0.006	3 × 10⁻⁵	176	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Carbenicillin methyl ester
AmpC beta-lactamasevariant T316A	Escherichia coli	carboxymethyl benzoylaminothioacetate	—	—	0.7	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, carboxymethyl benzoylaminothioacetate
AmpC beta-lactamasevariant T316A	Escherichia coli	Cefazolin	90	7.5	12	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cefazolin
AmpC beta-lactamasevariant T316A	Escherichia coli	Cefotaxime	0.077	0.00641	12	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cefotaxime
AmpC beta-lactamasevariant T316A	Escherichia coli	Cefuroxime	0.032	5.2 × 10⁻⁴	61	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cefuroxime
AmpC beta-lactamasevariant T316A	Escherichia coli	Cephalexin	15.6	0.105	150	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cephalexin
AmpC beta-lactamasevariant T316A	Escherichia coli	Cephaloridine	100	1.87	53	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cephaloridine
AmpC beta-lactamasevariant T316A	Escherichia coli	Cephalosporin C	216	0.36	600	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cephalosporin C
AmpC beta-lactamasevariant T316A	Escherichia coli	Cephalothin	175	0.93	188	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Cephalothin
AmpC beta-lactamasevariant T316A	Escherichia coli	deacetylcephalosporin C	512	0.223	2.3 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, deacetylcephalosporin C
AmpC beta-lactamasevariant T316A	Escherichia coli	Deacetylcephalosporin C lactone	139	0.0243	5.7 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Deacetylcephalosporin C lactone
AmpC beta-lactamasevariant T316A	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	—	—	0.19	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, DL-phenyl-lactyl benzoylaminoacetate
AmpC beta-lactamasevariant T316A	Escherichia coli	Imipenem	0.004	—	0.11	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Imipenem
AmpC beta-lactamasevariant T316A	Escherichia coli	Nitrocefin	645	0.658	980	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Nitrocefin
AmpC beta-lactamasevariant T316A	Escherichia coli	Phenoxymethylpenicillin	38	0.0168	2.27 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316A, Phenoxymethylpenicillin
AmpC beta-lactamasevariant T316V	Escherichia coli	(3-Hydroxymethyl)phenoxymethylpenicillin	1.13	0.00125	904	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, (3-Hydroxymethyl)phenoxymethylpenicillin
AmpC beta-lactamasevariant T316V	Escherichia coli	Ampicillin	0.82	0.0026	320	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Ampicillin
AmpC beta-lactamasevariant T316V	Escherichia coli	Aztreonam	0.0026	—	104	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Aztreonam
AmpC beta-lactamasevariant T316V	Escherichia coli	Benzylpenicillin	39	0.0101	3.8 × 10³	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Benzylpenicillin
AmpC beta-lactamasevariant T316V	Escherichia coli	Benzylpenicillin methyl ester	2.7	8 × 10⁻⁵	3.4 × 10⁴	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Benzylpenicillin methyl ester
AmpC beta-lactamasevariant T316V	Escherichia coli	Carbenicillin	0.0031	0.0078	0.4	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Carbenicillin
AmpC beta-lactamasevariant T316V	Escherichia coli	Carbenicillin methyl ester	0.0038	3 × 10⁻⁵	110	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Carbenicillin methyl ester
AmpC beta-lactamasevariant T316V	Escherichia coli	carboxymethyl benzoylaminothioacetate	—	—	0.24	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, carboxymethyl benzoylaminothioacetate
AmpC beta-lactamasevariant T316V	Escherichia coli	Cefazolin	—	—	3	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cefazolin
AmpC beta-lactamasevariant T316V	Escherichia coli	Cefotaxime	0.037	0.0328	1.1	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cefotaxime
AmpC beta-lactamasevariant T316V	Escherichia coli	Cefuroxime	0.025	0.003	8.3	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cefuroxime
AmpC beta-lactamasevariant T316V	Escherichia coli	Cephalexin	6.5	0.6	10.8	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cephalexin
AmpC beta-lactamasevariant T316V	Escherichia coli	Cephaloridine	—	—	8	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cephaloridine
AmpC beta-lactamasevariant T316V	Escherichia coli	Cephalosporin C	94	1.65	57	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cephalosporin C
AmpC beta-lactamasevariant T316V	Escherichia coli	Cephalothin	46	2.54	1.8	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Cephalothin
AmpC beta-lactamasevariant T316V	Escherichia coli	deacetylcephalosporin C	152	0.34	450	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, deacetylcephalosporin C
AmpC beta-lactamasevariant T316V	Escherichia coli	Deacetylcephalosporin C lactone	4	0.0061	660	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Deacetylcephalosporin C lactone
AmpC beta-lactamasevariant T316V	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	—	—	0.02	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, DL-phenyl-lactyl benzoylaminoacetate
AmpC beta-lactamasevariant T316V	Escherichia coli	Imipenem	0.003	—	0.034	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Imipenem
AmpC beta-lactamasevariant T316V	Escherichia coli	Nitrocefin	36	1.1	33	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Nitrocefin
AmpC beta-lactamasevariant T316V	Escherichia coli	Phenoxymethylpenicillin	14	0.015	910	pH 8, 30 °C	Open record: AmpC beta-lactamase variant T316V, Phenoxymethylpenicillin
TEM-1 beta-lactamase	Escherichia coli	(3-Hydroxymethyl)phenoxymethylpenicillin	285	1.8	160	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, (3-Hydroxymethyl)phenoxymethylpenicillin
TEM-1 beta-lactamase	Escherichia coli	Ampicillin	880	0.076	1.16 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Ampicillin
TEM-1 beta-lactamase	Escherichia coli	Aztreonam	1	1.5	0.67	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Aztreonam
TEM-1 beta-lactamase	Escherichia coli	Benzylpenicillin	1.42 × 10³	0.025	5.7 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Benzylpenicillin
TEM-1 beta-lactamase	Escherichia coli	Benzylpenicillin methyl ester	—	—	5.6	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Benzylpenicillin methyl ester
TEM-1 beta-lactamase	Escherichia coli	Carbenicillin	110	0.009	1.2 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Carbenicillin
TEM-1 beta-lactamase	Escherichia coli	carboxymethyl benzoylaminothioacetate	20	3.8	5.2	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, carboxymethyl benzoylaminothioacetate
TEM-1 beta-lactamase	Escherichia coli	Cefazolin	140	0.38	370	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cefazolin
TEM-1 beta-lactamase	Escherichia coli	Cefotaxime	—	—	1.5	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cefotaxime
TEM-1 beta-lactamase	Escherichia coli	Cefuroxime	6	1.05	5.7	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cefuroxime
TEM-1 beta-lactamase	Escherichia coli	Cephalexin	15	1.19	13	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cephalexin
TEM-1 beta-lactamase	Escherichia coli	Cephaloridine	1.5 × 10³	0.67	2.24 × 10³	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cephaloridine
TEM-1 beta-lactamase	Escherichia coli	Cephalosporin C	28	0.414	67	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cephalosporin C
TEM-1 beta-lactamase	Escherichia coli	Cephalothin	95	0.166	570	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Cephalothin
TEM-1 beta-lactamase	Escherichia coli	deacetylcephalosporin C	11	0.53	21	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, deacetylcephalosporin C
TEM-1 beta-lactamase	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	0.1	1.92	0.052	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, DL-phenyl-lactyl benzoylaminoacetate
TEM-1 beta-lactamase	Escherichia coli	Nitrocefin	850	0.046	1.848 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Nitrocefin
TEM-1 beta-lactamase	Escherichia coli	Phenoxymethylpenicillin	410	0.006	6.9 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase, Phenoxymethylpenicillin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	(3-Hydroxymethyl)phenoxymethylpenicillin	345	3.29	105	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, (3-Hydroxymethyl)phenoxymethylpenicillin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Ampicillin	600	0.093	6.43 × 10³	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Ampicillin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Aztreonam	—	—	0.0064	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Aztreonam
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Benzylpenicillin	413	0.023	1.8 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Benzylpenicillin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Benzylpenicillin methyl ester	—	—	9.1	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Benzylpenicillin methyl ester
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Carbenicillin	33	0.067	490	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Carbenicillin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	carboxymethyl benzoylaminothioacetate	—	—	0.3	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, carboxymethyl benzoylaminothioacetate
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cefazolin	—	—	1.5	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cefazolin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cefotaxime	—	—	0.0056	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cefotaxime
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cefuroxime	—	—	0.0144	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cefuroxime
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cephalexin	—	—	0.149	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cephalexin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cephaloridine	33	4.6	7.1	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cephaloridine
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cephalosporin C	3.1	11.7	0.26	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cephalosporin C
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Cephalothin	14	6.64	2.1	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Cephalothin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	deacetylcephalosporin C	0.48	7.7	0.062	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, deacetylcephalosporin C
TEM-1 beta-lactamasevariant S235A	Escherichia coli	DL-phenyl-lactyl benzoylaminoacetate	—	—	0.004	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, DL-phenyl-lactyl benzoylaminoacetate
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Nitrocefin	—	—	195	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Nitrocefin
TEM-1 beta-lactamasevariant S235A	Escherichia coli	Phenoxymethylpenicillin	123	0.0055	2.2 × 10⁴	pH 7, 30 °C	Open record: TEM-1 beta-lactamase variant S235A, Phenoxymethylpenicillin