CatLogenzyme kinetics catalog

Values CatLog read from this paper

Specificity inversion of Ochrobactrum anthropi D-aminopeptidase to a D,D-carboxypeptidase with new penicillin binding activity by directed mutagenesis

PMID 16131658doi:10.1110/ps.051475305Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (28 values) from this paper: D-aminopeptidase (DAP) from Ochrobactrum anthropi.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 3.4.11.19, EC 3.4.16.4, EC 3.5.2.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
D-aminopeptidase (DAP)Ochrobactrum anthropiD-alanine p-nitroanilide5800.461.26 × 10³pH 8, 30 °COpen
D-aminopeptidase (DAP)variant 475G487Ochrobactrum anthropiD-alanine p-nitroanilide——9 × 10⁻⁴pH 8, 30 °COpen
D-aminopeptidase (DAP)variant 475G487Ochrobactrum anthropiNa,Na-Ac2-L-Lys-D-Ala-D-Ala0.092004.5 × 10⁻⁴pH 8, 37 °COpen
D-aminopeptidase (DAP)variant 475G487Ochrobactrum anthropinitrocefin0.00160.0290.055 calc.pH 7, 30 °COpen
D-aminopeptidase (DAP)variant 475G487 + DAP-BC-His (complemented)Ochrobactrum anthropiD-alanine p-nitroanilide330.5165pH 8, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropiD-alanine p-nitroanilide——6 × 10⁻⁴pH 8, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropiN-alpha-acetyl-L-lysyl-D-alanyl-D-alanine0.21850.0011pH 8, 37 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropiNa,Na-Ac2-L-Lys-D-Ala-D-Ala0.62490.013pH 8, 37 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropinitrocefin8 × 10⁻⁴——pH 7, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropinitrocefin—0.026—pH 7, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropinitrocefin0.00125——pH 7, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropinitrocefin—0.025—pH 7, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropinitrocefin0.0011——pH 7, 30 °COpen
D-aminopeptidase (DAP)variant 475G487-N275ROchrobactrum anthropinitrocefin—0.035—pH 7, 30 °COpen
D-aminopeptidase (DAP)variant DAP-A + DAP-BC-His complex (coexpressed fragments)Ochrobactrum anthropiD-alanine p-nitroanilide2105.142pH 8, 30 °COpen