Values CatLog read from this paper
Purification and characterization of PBP4a, a new low-molecular-weight penicillin-binding protein from Bacillus subtilis
PMID 11160090doi:10.1128/jb.183.5.1595-1599.2001Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (17 values) from this paper: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), R39 DD-peptidase from Bacillus subtilis, Actinomadura sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase) | Bacillus subtilis | benzoyl-D-Ala-thioglycolate | 23 | 0.38 | 61 | pH 7.5, 30 °C | Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-D-Ala-thioglycolate |
| PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase) | Bacillus subtilis | benzoyl-D-Ala-thioglycolate10 mM D-alanine; cosubstrate D-alanine (10 mM) | 28 | 0.43 | 65 | pH 7.5, 30 °C | Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-D-Ala-thioglycolate |
| PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase) | Bacillus subtilis | benzoyl-D-Ala-thioglycolate50 mM D-alanine; cosubstrate D-alanine (50 mM) | 42 | 0.54 | 78 | pH 7.5, 30 °C | Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-D-Ala-thioglycolate |
| PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase) | Bacillus subtilis | benzoyl-Gly-thioglycolate | 2 | 0.47 | 4.3 | pH 7.5, 30 °C | Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), benzoyl-Gly-thioglycolate |
| PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase) | Bacillus subtilis | Nalpha-acetyl-L-Lys-D-Ala-D-Ala | — | — | 6.7 | pH 7.5, 30 °C | Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), Nalpha-acetyl-L-Lys-D-Ala-D-Ala |
| PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase) | Bacillus subtilis | Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate | — | — | 35 | pH 7.5, 30 °C | Open record: PBP4a (penicillin-binding protein 4a, DD-carboxypeptidase), Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate |
| R39 DD-peptidase | Actinomadura sp. | Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate | 5.4 | 0.042 | 130 | pH 7.5, 30 °C | Open record: R39 DD-peptidase, Nalpha-acetyl-L-Lys-D-Ala-D-thiolactate |