Values CatLog read from this paper
Catalytic mechanism of the Streptomyces K15 DD-transpeptidase/penicillin-binding protein probed by site-directed mutagenesis and structural analysis
PMID 12627955doi:10.1021/bi027256xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (51 values) from this paper: Streptomyces K15 DD-transpeptidase/penicillin-binding protein from Streptomyces sp. K15.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | benzoyl-D-alanyl-thioglycolate | 0.3 | 1.3 | 0.2 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, benzoyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | benzoyl-glycyl-thioglycolate | 0.08 | 0.8 | 0.1 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, benzoyl-glycyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | benzoyl-glycyl-thiolactate | 0.08 | 0.3 | 0.27 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, benzoyl-glycyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine | — | — | 0.006 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate | 1.1 | 2 | 0.58 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine | 0.13 | 30 | 0.0043 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate | 0.8 | 0.6 | 1.3 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thioglycolate | — | — | 0.29 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, phenylacetyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98A | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thiolactate | 2.2 | 1.2 | 2 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98A, phenylacetyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | benzoyl-D-alanyl-thioglycolate | — | — | 0.26 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, benzoyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | benzoyl-glycyl-thioglycolate | — | — | 0.085 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, benzoyl-glycyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | benzoyl-glycyl-thiolactate | 0.45 | 3.3 | 0.14 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, benzoyl-glycyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine | — | — | 0.001 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate | — | — | 0.19 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine | — | — | 7 × 10⁻⁴ | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate | — | — | 0.29 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thioglycolate | — | — | 0.32 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, phenylacetyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant C98N | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thiolactate | — | — | 0.8 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant C98N, phenylacetyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H | Streptomyces sp. K15 | benzoyl-D-alanyl-thioglycolate | — | — | 0.005 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, benzoyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H | Streptomyces sp. K15 | benzoyl-glycyl-thiolactate | 0.006 | 1 | 0.006 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, benzoyl-glycyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thioglycolate | 0.015 | 2.4 | 0.0064 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, phenylacetyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant K38H | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thiolactate | — | — | 0.0011 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant K38H, phenylacetyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | benzoyl-D-alanyl-thioglycolate | — | — | 0.004 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, benzoyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | benzoyl-glycyl-thioglycolate | — | — | 0.0024 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, benzoyl-glycyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | benzoyl-glycyl-thiolactate | — | — | 0.015 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, benzoyl-glycyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine | — | — | 2.7 × 10⁻⁴ | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate | — | — | 0.0068 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine | — | — | 1.5 × 10⁻⁴ | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha-acetyl-L-lysyl-D-alanyl-D-alanine |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate | — | — | 0.0076 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, Nalpha-acetyl-L-lysyl-D-alanyl-thiolactate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thioglycolate | — | — | 0.008 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, phenylacetyl-D-alanyl-thioglycolate |
| Streptomyces K15 DD-transpeptidase/penicillin-binding proteinvariant S96A | Streptomyces sp. K15 | phenylacetyl-D-alanyl-thiolactate | — | — | 0.004 | pH 7.2, 37 °C | Open record: Streptomyces K15 DD-transpeptidase/penicillin-binding protein variant S96A, phenylacetyl-D-alanyl-thiolactate |