EC 2.7.1.130: tetraacyldisaccharide 4'-kinase
TransferasesOpen in Browse
CatLog read 37 records for EC 2.7.1.130 from 2 papers, for 1 organism. Names in these papers: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 2 records for EC 2.7.1.130 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.1.130 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Mechanistic characterization of the tetraacyldisaccharide-1-phosphate 4'-kinase LpxK involved in lipid A biosynthesis | Aquifex aeolicus | 22 |
| Structural basis of lipid binding for the membrane-embedded tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | 15 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 2.4 | 0.007 | 340 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK), tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant D138A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.00106 | 0.047 | 0.023 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant D138A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant D138N | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.028 | 0.007 | 4.0 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant D138N, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant D139A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 5.1 × 10⁻⁴ | 0.046 | 0.011 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant D139A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant D139N | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.0063 | 0.014 | 0.45 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant D139N, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant E100A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.00123 | 0.0174 | 0.0707 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant E100A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant E172A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.7 | 0.018 | 39 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant E172A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant H143A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.16 | 0.05 | 3.2 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant H143A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant H261A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.0031 | 0.015 | 0.21 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant H261A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant N43A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.36 | 0.009 | 40 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant N43A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant Q142A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.35 | 0.028 | 12 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant Q142A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant R119A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.036 | 0.0097 | 3.7 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant R119A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant R171A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.0138 | 0.128 | 0.108 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant R171A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant R72A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.39 | 0.028 | 14 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant R72A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK)variant Y74A | Aquifex aeolicus | tetraacyldisaccharide-1-phosphate | 0.031 | 0.048 | 0.65 calc. | pH 8.5 | PMID 25023290 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase (LpxK) variant Y74A, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | adenosine triphosphateATP and MgCl2 varied 1:1 (0.3-10 mM) at fixed 50 µM DSMP | 12.3 | 1.6 | 7.7 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | adenosine triphosphateglobal fit to sequential mechanism (Eq. 3, PRISM); purified | 18 | 1 | 18 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | adenosine triphosphateMichaelis-Menten fit; cosubstrate DSMP (50 uM) | 3.9 | 1.2 | 3.2 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | tetraacyldisaccharide-1-phosphateDSMP varied 1.56-100 µM at fixed 5 mM ATP/MgCl2 | 9.2 | 0.007 | 1.3 × 10³ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | tetraacyldisaccharide-1-phosphateglobal fit to sequential mechanism (Eq. 3, PRISM) | — | 0.007 | — | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D138A | Aquifex aeolicus | adenosine triphosphate | 8.3 × 10⁻⁴ | 4 | 2.1 × 10⁻⁴ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D138A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D138N | Aquifex aeolicus | adenosine triphosphate | 0.057 | 3.2 | 0.018 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D138N, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D139A | Aquifex aeolicus | adenosine triphosphate | 4.8 × 10⁻⁴ | 5 | 9.6 × 10⁻⁵ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D139A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D139N | Aquifex aeolicus | adenosine triphosphate | 0.0076 | 1.4 | 0.0054 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D139N, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D260A | Aquifex aeolicus | adenosine triphosphate | 0.073 | 1.6 | 0.046 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D260A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D99A | Aquifex aeolicus | adenosine triphosphate | 0.0015 | 2.8 | 5.4 × 10⁻⁴ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D99A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D99E | Aquifex aeolicus | adenosine triphosphate | 0.05 | 2.3 | 0.022 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D99E, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D99N | Aquifex aeolicus | adenosine triphosphate | 3.5 × 10⁻⁴ | 1.1 | 3.2 × 10⁻⁴ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D99N, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant E100A | Aquifex aeolicus | adenosine triphosphate | 0.0029 | 1.9 | 0.0015 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant E100A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant E100D | Aquifex aeolicus | adenosine triphosphate | 0.0027 | 2 | 0.0014 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant E100D, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant E100Q | Aquifex aeolicus | adenosine triphosphate | 4.8 × 10⁻⁴ | 2.9 | 1.7 × 10⁻⁴ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant E100Q, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant H261A | Aquifex aeolicus | adenosine triphosphate | 0.0046 | 1.3 | 0.0035 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant H261A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant K51A | Aquifex aeolicus | adenosine triphosphate | 0.0013 | 1.5 | 8.7 × 10⁻⁴ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant K51A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant S49A | Aquifex aeolicus | adenosine triphosphate | 3.9 | 2.7 | 1.4 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant S49A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant S53A | Aquifex aeolicus | adenosine triphosphate | 0.47 | 6 | 0.078 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant S53A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant T52A | Aquifex aeolicus | adenosine triphosphate | 0.0013 | 2.2 | 5.9 × 10⁻⁴ calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant T52A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant Y74A | Aquifex aeolicus | adenosine triphosphate | 0.022 | 1.7 | 0.013 calc. | pH 8.5, 30 °C | PMID 23464738 | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant Y74A, adenosine triphosphate |