Values CatLog read from this paper
Mechanistic characterization of the tetraacyldisaccharide-1-phosphate 4'-kinase LpxK involved in lipid A biosynthesis
PMID 23464738doi:10.1021/bi400097zOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (43 values) from this paper: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK from Aquifex aeolicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 21 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | adenosine triphosphateATP and MgCl2 varied 1:1 (0.3-10 mM) at fixed 50 µM DSMP | 12.3 | 1.6 | 7.7 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | adenosine triphosphateglobal fit to sequential mechanism (Eq. 3, PRISM); purified | 18 | 1 | 18 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | adenosine triphosphateMichaelis-Menten fit; cosubstrate DSMP (50 uM) | 3.9 | 1.2 | 3.2 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | tetraacyldisaccharide-1-phosphateDSMP varied 1.56-100 µM at fixed 5 mM ATP/MgCl2 | 9.2 | 0.007 | 1.3 × 10³ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxK | Aquifex aeolicus | tetraacyldisaccharide-1-phosphateglobal fit to sequential mechanism (Eq. 3, PRISM) | — | 0.007 | — | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK, tetraacyldisaccharide-1-phosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D138A | Aquifex aeolicus | adenosine triphosphate | 8.3 × 10⁻⁴ | 4 | 2.1 × 10⁻⁴ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D138A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D138N | Aquifex aeolicus | adenosine triphosphate | 0.057 | 3.2 | 0.018 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D138N, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D139A | Aquifex aeolicus | adenosine triphosphate | 4.8 × 10⁻⁴ | 5 | 9.6 × 10⁻⁵ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D139A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D139N | Aquifex aeolicus | adenosine triphosphate | 0.0076 | 1.4 | 0.0054 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D139N, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D260A | Aquifex aeolicus | adenosine triphosphate | 0.073 | 1.6 | 0.046 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D260A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D99A | Aquifex aeolicus | adenosine triphosphate | 0.0015 | 2.8 | 5.4 × 10⁻⁴ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D99A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D99E | Aquifex aeolicus | adenosine triphosphate | 0.05 | 2.3 | 0.022 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D99E, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant D99N | Aquifex aeolicus | adenosine triphosphate | 3.5 × 10⁻⁴ | 1.1 | 3.2 × 10⁻⁴ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant D99N, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant E100A | Aquifex aeolicus | adenosine triphosphate | 0.0029 | 1.9 | 0.0015 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant E100A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant E100D | Aquifex aeolicus | adenosine triphosphate | 0.0027 | 2 | 0.0014 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant E100D, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant E100Q | Aquifex aeolicus | adenosine triphosphate | 4.8 × 10⁻⁴ | 2.9 | 1.7 × 10⁻⁴ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant E100Q, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant H261A | Aquifex aeolicus | adenosine triphosphate | 0.0046 | 1.3 | 0.0035 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant H261A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant K51A | Aquifex aeolicus | adenosine triphosphate | 0.0013 | 1.5 | 8.7 × 10⁻⁴ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant K51A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant S49A | Aquifex aeolicus | adenosine triphosphate | 3.9 | 2.7 | 1.4 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant S49A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant S53A | Aquifex aeolicus | adenosine triphosphate | 0.47 | 6 | 0.078 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant S53A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant T52A | Aquifex aeolicus | adenosine triphosphate | 0.0013 | 2.2 | 5.9 × 10⁻⁴ calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant T52A, adenosine triphosphate |
| tetraacyldisaccharide-1-phosphate 4'-kinase LpxKvariant Y74A | Aquifex aeolicus | adenosine triphosphate | 0.022 | 1.7 | 0.013 calc. | pH 8.5, 30 °C | Open record: tetraacyldisaccharide-1-phosphate 4'-kinase LpxK variant Y74A, adenosine triphosphate |