EC 2.7.1.160: 2'-phosphotransferase

TransferasesOpen in Browse

CatLog read 14 records for EC 2.7.1.160 from 1 paper, for 1 organism. Names in these papers: 2'-phosphotransferase Tpt1.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

Papers with records for EC 2.7.1.160
Paper	Organisms	Records
Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog	Saccharomyces cerevisiae	14

Records

Records for EC 2.7.1.160 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)30-min reactions with 5'-32P-labeled RNA	—	3.5 × 10⁻⁷	1.4 × 10⁴	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)4.25 pM Tpt1; ligated tRNA varied (Figure 2)	0.0022	3.5 × 10⁻⁷	6.2 × 10³ calc.	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated octadecamer pApCpApApGpApCpUpGpUpApA(2′p)pApUpCpUpUpG (anticodon stem-loop of tRNA^Tyr)	0.04	1.7 × 10⁻⁶	2.4 × 10⁴	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated octadecamer pApCpApApGpApCpUpGpUpApA(2′p)pApUpCpUpUpG (anticodon stem-loop of tRNA^Tyr)
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated octamer pGpUpApA(2′p)pApUpCpU	0.32	2 × 10⁻⁵	1.6 × 10⁴	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated octamer pGpUpApA(2′p)pApUpCpU
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+1.1 nM ligated tRNA (nearly saturating); 42.5 pM Tpt1	—	0.01	—	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+3.5 µM pApAppA (nearly saturating); 42.5 pM Tpt1	—	0.2	—	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+11 µM pApApA^p (subsaturating); data not shown	—	0.66	—	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+105 nM pApAppA (subsaturating); data not shown	—	0.1	—	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApA(2′p)pA, trinucleotide with an internal 2′-phosphate	0.73	1.5 × 10⁻⁴	5 × 10³	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, pApA(2′p)pA, trinucleotide with an internal 2′-phosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApApA(2′p), trinucleotide with a terminal 2′-phosphate flanked by a 3′-hydroxyl	0.0057	0.04	0.14	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, pApApA(2′p), trinucleotide with a terminal 2′-phosphate flanked by a 3′-hydroxyl
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApApA(2′p)p, terminal 2′-phosphate adjacent to a 3′-phosphate	0.15	6.5 × 10⁻⁴	230	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, pApApA(2′p)p, terminal 2′-phosphate adjacent to a 3′-phosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApApA(2′p)pOCH3, terminal 2′-phosphate adjacent to a 3′-methoxyphosphate	1.173	0.003	390	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, pApApA(2′p)pOCH3, terminal 2′-phosphate adjacent to a 3′-methoxyphosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApU(2′p)pU, trinucleotide with an internal 2′-phosphate flanked by uridines	0.45	4.8 × 10⁻⁴	940	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, pApU(2′p)pU, trinucleotide with an internal 2′-phosphate flanked by uridines
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pUpU(2′p)pU, trinucleotide with an internal 2′-phosphate	0.147	3.25 × 10⁻⁴	450	30 °C	PMID 11705403	Open record: 2'-phosphotransferase Tpt1, pUpU(2′p)pU, trinucleotide with an internal 2′-phosphate

CatLog snapshot Oct 24, 2026 (data ID 660092338b). CatLog's compilation is free to reuse under CC BY 4.0; records from a database keep its license. How to cite.