Values CatLog read from this paper

Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog

PMID 11705403doi:10.1021/bi011388tOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 23 records (41 values) from this paper: 2'-phosphotransferase Tpt1, 2'-phosphotransferase KptA from Saccharomyces cerevisiae, Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 2.7.1.160

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
2'-phosphotransferase KptA	Escherichia coli	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)1 mM NAD (not saturating)	—	4 × 10⁻⁶	—	37 °C	Open record: 2'-phosphotransferase KptA, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase KptA	Escherichia coli	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)1 mM NAD; saturating ligated tRNA; 30-min reactions	0.00108	—	—	37 °C	Open record: 2'-phosphotransferase KptA, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase KptA	Escherichia coli	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)20 mM NAD; saturating ligated tRNA; 30-min reactions	0.0025	—	—	37 °C	Open record: 2'-phosphotransferase KptA, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase KptA	Escherichia coli	2′-phosphorylated octadecamer pApCpApApGpApCpUpGpUpApA(2′p)pApUpCpUpUpG (anticodon stem-loop of tRNA^Tyr)	—	1.5 × 10⁻⁴	—	37 °C	Open record: 2'-phosphotransferase KptA, 2′-phosphorylated octadecamer pApCpApApGpApCpUpGpUpApA(2′p)pApUpCpUpUpG (anticodon stem-loop of tRNA^Tyr)
2'-phosphotransferase KptA	Escherichia coli	2′-phosphorylated octamer pGpUpApA(2′p)pApUpCpU	—	2.5 × 10⁻⁴	—	37 °C	Open record: 2'-phosphotransferase KptA, 2′-phosphorylated octamer pGpUpApA(2′p)pApUpCpU
2'-phosphotransferase KptA	Escherichia coli	pApA(2′p)pA, trinucleotide with an internal 2′-phosphate	—	7 × 10⁻⁴	—	37 °C	Open record: 2'-phosphotransferase KptA, pApA(2′p)pA, trinucleotide with an internal 2′-phosphate
2'-phosphotransferase KptA	Escherichia coli	pApApA(2′p)p, terminal 2′-phosphate adjacent to a 3′-phosphate	—	0.0013	—	37 °C	Open record: 2'-phosphotransferase KptA, pApApA(2′p)p, terminal 2′-phosphate adjacent to a 3′-phosphate
2'-phosphotransferase KptA	Escherichia coli	pApApA(2′p)pOCH3, terminal 2′-phosphate adjacent to a 3′-methoxyphosphate	—	0.0038	—	37 °C	Open record: 2'-phosphotransferase KptA, pApApA(2′p)pOCH3, terminal 2′-phosphate adjacent to a 3′-methoxyphosphate
2'-phosphotransferase KptA	Escherichia coli	pUpU(2′p)pU, trinucleotide with an internal 2′-phosphate	—	0.0018	—	37 °C	Open record: 2'-phosphotransferase KptA, pUpU(2′p)pU, trinucleotide with an internal 2′-phosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)30-min reactions with 5'-32P-labeled RNA	—	3.5 × 10⁻⁷	1.4 × 10⁴	30 °C	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)4.25 pM Tpt1; ligated tRNA varied (Figure 2)	0.0022	3.5 × 10⁻⁷	6.2 × 10³ calc.	30 °C	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated ligated yeast tRNA^Phe (splice-junction 2′-phosphate)
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated octadecamer pApCpApApGpApCpUpGpUpApA(2′p)pApUpCpUpUpG (anticodon stem-loop of tRNA^Tyr)	0.04	1.7 × 10⁻⁶	2.4 × 10⁴	30 °C	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated octadecamer pApCpApApGpApCpUpGpUpApA(2′p)pApUpCpUpUpG (anticodon stem-loop of tRNA^Tyr)
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	2′-phosphorylated octamer pGpUpApA(2′p)pApUpCpU	0.32	2 × 10⁻⁵	1.6 × 10⁴	30 °C	Open record: 2'-phosphotransferase Tpt1, 2′-phosphorylated octamer pGpUpApA(2′p)pApUpCpU
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+1.1 nM ligated tRNA (nearly saturating); 42.5 pM Tpt1	—	0.01	—	30 °C	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+3.5 µM pApAppA (nearly saturating); 42.5 pM Tpt1	—	0.2	—	30 °C	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+11 µM pApApA^p (subsaturating); data not shown	—	0.66	—	30 °C	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	NAD+105 nM pApAppA (subsaturating); data not shown	—	0.1	—	30 °C	Open record: 2'-phosphotransferase Tpt1, NAD+
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApA(2′p)pA, trinucleotide with an internal 2′-phosphate	0.73	1.5 × 10⁻⁴	5 × 10³	30 °C	Open record: 2'-phosphotransferase Tpt1, pApA(2′p)pA, trinucleotide with an internal 2′-phosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApApA(2′p), trinucleotide with a terminal 2′-phosphate flanked by a 3′-hydroxyl	0.0057	0.04	0.14	30 °C	Open record: 2'-phosphotransferase Tpt1, pApApA(2′p), trinucleotide with a terminal 2′-phosphate flanked by a 3′-hydroxyl
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApApA(2′p)p, terminal 2′-phosphate adjacent to a 3′-phosphate	0.15	6.5 × 10⁻⁴	230	30 °C	Open record: 2'-phosphotransferase Tpt1, pApApA(2′p)p, terminal 2′-phosphate adjacent to a 3′-phosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApApA(2′p)pOCH3, terminal 2′-phosphate adjacent to a 3′-methoxyphosphate	1.173	0.003	390	30 °C	Open record: 2'-phosphotransferase Tpt1, pApApA(2′p)pOCH3, terminal 2′-phosphate adjacent to a 3′-methoxyphosphate
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pApU(2′p)pU, trinucleotide with an internal 2′-phosphate flanked by uridines	0.45	4.8 × 10⁻⁴	940	30 °C	Open record: 2'-phosphotransferase Tpt1, pApU(2′p)pU, trinucleotide with an internal 2′-phosphate flanked by uridines
2'-phosphotransferase Tpt1	Saccharomyces cerevisiae	pUpU(2′p)pU, trinucleotide with an internal 2′-phosphate	0.147	3.25 × 10⁻⁴	450	30 °C	Open record: 2'-phosphotransferase Tpt1, pUpU(2′p)pU, trinucleotide with an internal 2′-phosphate