EC 2.7.1.95: kanamycin kinase
TransferasesOpen in Browse
CatLog read 11 records for EC 2.7.1.95 from 1 paper, for 1 organism. Names in these papers: aminoglycoside 3'-phosphotransferase APH(3')-IIIa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 8 records for EC 2.7.1.95 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.1.95 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Broad spectrum aminoglycoside phosphotransferase type III from Enterococcus: overexpression, purification, and substrate specificity | Enterococcus faecalis | 11 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | amikacin | 2.46 | 0.245 | 10 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, amikacin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | ATP | 1.76 | 0.0277 | 63.7 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, ATP |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | butirosin | 2.02 | 0.0343 | 58.7 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, butirosin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycinmonomer form (Mono Q peak 1 / Superdex 200, ~30 kDa) | 1.63 | 0.018 | 91 calc. | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycindimer form (Mono Q peak 2 / Superdex 200, ~60 kDa) | 1.6 | 0.0163 | 98 calc. | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycin A | 1.79 | 0.0126 | 143 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin A |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycin B | 3.51 | 0.0194 | 181 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin B |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | lividomycin A | 3.97 | 0.0316 | 126 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, lividomycin A |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | neomycin B | 2.08 | 0.00772 | 269 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, neomycin B |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | paromomycin | 3.62 | 0.0195 | 186 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, paromomycin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | ribostamycin | 1.89 | 0.0093 | 203 | pH 7.5, 37 °C | PMID 8204627 | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, ribostamycin |