Values CatLog read from this paper
Broad spectrum aminoglycoside phosphotransferase type III from Enterococcus: overexpression, purification, and substrate specificity
PMID 8204627doi:10.1021/bi00188a024Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (31 values) from this paper: aminoglycoside 3'-phosphotransferase APH(3')-IIIa from Enterococcus faecalis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | amikacin | 2.46 | 0.245 | 10 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, amikacin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | ATP | 1.76 | 0.0277 | 63.7 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, ATP |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | butirosin | 2.02 | 0.0343 | 58.7 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, butirosin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycinmonomer form (Mono Q peak 1 / Superdex 200, ~30 kDa) | 1.63 | 0.018 | 91 calc. | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycindimer form (Mono Q peak 2 / Superdex 200, ~60 kDa) | 1.6 | 0.0163 | 98 calc. | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycin A | 1.79 | 0.0126 | 143 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin A |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | kanamycin B | 3.51 | 0.0194 | 181 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, kanamycin B |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | lividomycin A | 3.97 | 0.0316 | 126 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, lividomycin A |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | neomycin B | 2.08 | 0.00772 | 269 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, neomycin B |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | paromomycin | 3.62 | 0.0195 | 186 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, paromomycin |
| aminoglycoside 3'-phosphotransferase APH(3')-IIIa | Enterococcus faecalis | ribostamycin | 1.89 | 0.0093 | 203 | pH 7.5, 37 °C | Open record: aminoglycoside 3'-phosphotransferase APH(3')-IIIa, ribostamycin |