CatLogenzyme kinetics catalog

EC 2.7.7.40: D-ribitol-5-phosphate cytidylyltransferase

TransferasesOpen in Browse

CatLog read 29 records for EC 2.7.7.40 from 3 papers, for 2 organisms. Names in these papers: CDP-ribitol synthase (Bcs1), Bcs1 (bifunctional CDP-ribitol synthase), ribitol 5-phosphate cytidylyltransferase activity, Bcs1 (bifunctional CDP-ribitol synthase), ribulose 5-phosphate reductase activity, TarI'J' (CDP-ribitol synthase) and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 1 record for EC 2.7.7.40 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.40 in Browse.

Papers

PaperOrganismsRecords
Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzaeHaemophilus influenzae21
Bifunctional catalysis by CDP-ribitol synthase: convergent recruitment of reductase and cytidylyltransferase activities in Haemophilus influenzae and Staphylococcus aureusHaemophilus influenzae, Staphylococcus aureus6
Duplication of teichoic acid biosynthetic genes in Staphylococcus aureus leads to functionally redundant poly(ribitol phosphate) polymerasesStaphylococcus aureus2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
Bcs1 (bifunctional CDP-ribitol synthase), ribitol 5-phosphate cytidylyltransferase activityHaemophilus influenzaecytidine triphosphate23.50.008512.76 × 10³PMID 15362865Open
Bcs1 (bifunctional CDP-ribitol synthase), ribitol 5-phosphate cytidylyltransferase activityHaemophilus influenzaeD-ribitol 5-phosphate—0.01471.59 × 10³PMID 15362865Open
Bcs1 (bifunctional CDP-ribitol synthase), ribulose 5-phosphate reductase activityHaemophilus influenzaeD-ribulose 5-phosphate—0.10670.2PMID 15362865Open
Bcs1 (bifunctional CDP-ribitol synthase), ribulose 5-phosphate reductase activityHaemophilus influenzaeNADPH7.410.007061.05 × 10³PMID 15362865Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeCTPC-terminally His6-tagged Bcs1 (pET22b-bcs1-His)130.099130pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeCTPnative untagged Bcs1 (no His tag, recombinant, pET22b-bcs1)—0.074—pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-arabinose 5-phosphate2.330.77pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-erythrose 4-phosphate0.50.03813pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribitol 5-phosphateC-terminally His6-tagged Bcs1 (pET22b-bcs1-His)130.13100pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribitol 5-phosphatenative untagged Bcs1 (no His tag, recombinant, pET22b-bcs1)120.075160 calc.pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribose 5-phosphate4.12.21.9pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribulose3.61.13.3pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribulose 5-phosphate85 µM CTP; 10 mM MgCl2; apparent Km; apparent kcat/Km220.076290pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribulose 5-phosphate85 µM CTP; 10 mM MgCl2; apparent Km260.042620 calc.pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeD-ribulose 5-phosphatewithout the CTP (85 µM) and MgCl2 (10 mM) used for Table 111——pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeNADPHC-terminally His6-tagged Bcs1 (pET22b-bcs1-His)220.044500pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)Haemophilus influenzaeNADPHnative untagged Bcs1 (no His tag, recombinant, pET22b-bcs1)—0.025—pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant K386AHaemophilus influenzaeCTP—0.092110pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant K386AHaemophilus influenzaeD-ribitol 5-phosphate9.70.1281pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant K386AHaemophilus influenzaeD-ribulose 5-phosphate0.320.191.7pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant K386AHaemophilus influenzaeNADPH—0.0398.2pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant R18AHaemophilus influenzaeCTP—0.390.36pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant R18AHaemophilus influenzaeD-ribitol 5-phosphate0.1410.14pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant R18AHaemophilus influenzaeD-ribulose 5-phosphate6.80.2725pH 7.2, 25 °CPMID 11305920Open
CDP-ribitol synthase (Bcs1)variant R18AHaemophilus influenzaeNADPH—0.017400pH 7.2, 25 °CPMID 11305920Open
TarI'J' (CDP-ribitol synthase)Staphylococcus aureusCTP66.70.03871.72 × 10³PMID 18556787Open
TarI'J' (CDP-ribitol synthase)Staphylococcus aureusribitol-5-phosphate—0.1022653PMID 18556787Open
TarIJ (TarI-TarJ CDP-ribitol synthase complex), ribitol 5-phosphate cytidylyltransferase activity (TarI)Staphylococcus aureuscytidine triphosphate76.80.0789973PMID 15362865Open
TarIJ (TarI-TarJ CDP-ribitol synthase complex), ribitol 5-phosphate cytidylyltransferase activity (TarI)Staphylococcus aureusD-ribitol 5-phosphate—1.2859.8PMID 15362865Open