Values CatLog read from this paper
Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae
PMID 11305920doi:10.1021/bi002745nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 21 records (51 values) from this paper: CDP-ribitol synthase (Bcs1) from Haemophilus influenzae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | CTPC-terminally His6-tagged Bcs1 (pET22b-bcs1-His) | 13 | 0.099 | 130 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), CTP |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | CTPnative untagged Bcs1 (no His tag, recombinant, pET22b-bcs1) | — | 0.074 | — | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), CTP |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-arabinose 5-phosphate | 2.3 | 3 | 0.77 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-arabinose 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-erythrose 4-phosphate | 0.5 | 0.038 | 13 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-erythrose 4-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribitol 5-phosphateC-terminally His6-tagged Bcs1 (pET22b-bcs1-His) | 13 | 0.13 | 100 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribitol 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribitol 5-phosphatenative untagged Bcs1 (no His tag, recombinant, pET22b-bcs1) | 12 | 0.075 | 160 calc. | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribitol 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribose 5-phosphate | 4.1 | 2.2 | 1.9 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribose 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose | 3.6 | 1.1 | 3.3 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribulose |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphate85 µM CTP; 10 mM MgCl2; apparent Km; apparent kcat/Km | 22 | 0.076 | 290 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribulose 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphate85 µM CTP; 10 mM MgCl2; apparent Km | 26 | 0.042 | 620 calc. | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribulose 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphatewithout the CTP (85 µM) and MgCl2 (10 mM) used for Table 1 | 11 | — | — | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), D-ribulose 5-phosphate |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | NADPHC-terminally His6-tagged Bcs1 (pET22b-bcs1-His) | 22 | 0.044 | 500 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), NADPH |
| CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | NADPHnative untagged Bcs1 (no His tag, recombinant, pET22b-bcs1) | — | 0.025 | — | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1), NADPH |
| CDP-ribitol synthase (Bcs1)variant K386A | Haemophilus influenzae | CTP | — | 0.092 | 110 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant K386A, CTP |
| CDP-ribitol synthase (Bcs1)variant K386A | Haemophilus influenzae | D-ribitol 5-phosphate | 9.7 | 0.12 | 81 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant K386A, D-ribitol 5-phosphate |
| CDP-ribitol synthase (Bcs1)variant K386A | Haemophilus influenzae | D-ribulose 5-phosphate | 0.32 | 0.19 | 1.7 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant K386A, D-ribulose 5-phosphate |
| CDP-ribitol synthase (Bcs1)variant K386A | Haemophilus influenzae | NADPH | — | 0.039 | 8.2 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant K386A, NADPH |
| CDP-ribitol synthase (Bcs1)variant R18A | Haemophilus influenzae | CTP | — | 0.39 | 0.36 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant R18A, CTP |
| CDP-ribitol synthase (Bcs1)variant R18A | Haemophilus influenzae | D-ribitol 5-phosphate | 0.14 | 1 | 0.14 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant R18A, D-ribitol 5-phosphate |
| CDP-ribitol synthase (Bcs1)variant R18A | Haemophilus influenzae | D-ribulose 5-phosphate | 6.8 | 0.27 | 25 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant R18A, D-ribulose 5-phosphate |
| CDP-ribitol synthase (Bcs1)variant R18A | Haemophilus influenzae | NADPH | — | 0.017 | 400 | pH 7.2, 25 °C | Open record: CDP-ribitol synthase (Bcs1) variant R18A, NADPH |