CatLogenzyme kinetics catalog

EC 2.7.7.59: [protein-PII] uridylyltransferase

TransferasesOpen in Browse

CatLog read 15 records for EC 2.7.7.59 from 1 paper, for 1 organism. Names in these papers: uridylyltransferase/uridylyl-removing enzyme (UTase/UR).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

Papers

PaperOrganismsRecords
Enzymological characterization of the signal-transducing uridylyltransferase/uridylyl-removing enzyme (EC 2.7.7.59) of Escherichia coli and its interaction with the PII proteinEscherichia coli15

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliadenosine 5'-triphosphate0.4921.870.263 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia colicytidine 5'-triphosphate1.0750.382.8 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliguanosine 5'-triphosphate0.330.940.35 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliPII signal transduction protein25 mM MgCl2; 4 nM UTase/UR; PII and UTP both varied2.280.003760 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliPII signal transduction protein0.5 mM ATP; no 2-ketoglutarate (Table 2 footnote a)0.50.0019260 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliPII signal transduction protein0.5 mM ATP; no 2-ketoglutarate (Table 2 footnote a)0.420.0029140 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridine 5'-triphosphate100 mM KCl; 25 mM MgCl2; 1 mM DTT; 0.3 mg/mL BSA—0.04—pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridine 5'-triphosphate0.5 mM ATP; no 2-ketoglutarate (Table 2 footnote a)0.370.020318 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridine 5'-triphosphatealpha-32P label on the varied nucleotide; PII fixed1.4580.0436 calc.pH 7.5, 30 °CPMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridylylated PII signal transduction protein (PII-UMP)50 mM MgCl2; no glutamine0.0450.002320 calc.pH 7.5PMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridylylated PII signal transduction protein (PII-UMP)50 mM MgCl2; 2.5 mM glutamine (saturating activator)0.1088.2 × 10⁻⁴130 calc.pH 7.5PMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridylylated PII signal transduction protein (PII-UMP)50 mM MgCl2; 1 mM glutamine—0.0012—pH 7.5PMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridylylated PII signal transduction protein (PII-UMP)1 mM MnCl2; no MgCl2; 6 mM glutamine (saturating activator)1.023.2 × 10⁻⁴3.2 × 10³ calc.pH 7.5PMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridylylated PII signal transduction protein (PII-UMP)1 mM MnCl2; no MgCl2; no glutamine0.1556.3 × 10⁻⁴250 calc.pH 7.5PMID 9737855Open
uridylyltransferase/uridylyl-removing enzyme (UTase/UR)Escherichia coliuridylylated PII signal transduction protein (PII-UMP)0.033——pH 7.5, 30 °CPMID 9737855Open