Values CatLog read from this paper
Enzymological characterization of the signal-transducing uridylyltransferase/uridylyl-removing enzyme (EC 2.7.7.59) of Escherichia coli and its interaction with the PII protein
PMID 9737855doi:10.1021/bi980667mOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (27 values) from this paper: uridylyltransferase/uridylyl-removing enzyme (UTase/UR) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | adenosine 5'-triphosphate | 0.492 | 1.87 | 0.263 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), adenosine 5'-triphosphate |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | cytidine 5'-triphosphate | 1.075 | 0.38 | 2.8 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), cytidine 5'-triphosphate |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | guanosine 5'-triphosphate | 0.33 | 0.94 | 0.35 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), guanosine 5'-triphosphate |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | PII signal transduction protein25 mM MgCl2; 4 nM UTase/UR; PII and UTP both varied | 2.28 | 0.003 | 760 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), PII signal transduction protein |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | PII signal transduction protein0.5 mM ATP; no 2-ketoglutarate (Table 2 footnote a) | 0.5 | 0.0019 | 260 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), PII signal transduction protein |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | PII signal transduction protein0.5 mM ATP; no 2-ketoglutarate (Table 2 footnote a) | 0.42 | 0.0029 | 140 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), PII signal transduction protein |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridine 5'-triphosphate100 mM KCl; 25 mM MgCl2; 1 mM DTT; 0.3 mg/mL BSA | — | 0.04 | — | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridine 5'-triphosphate |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridine 5'-triphosphate0.5 mM ATP; no 2-ketoglutarate (Table 2 footnote a) | 0.37 | 0.0203 | 18 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridine 5'-triphosphate |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridine 5'-triphosphatealpha-32P label on the varied nucleotide; PII fixed | 1.458 | 0.04 | 36 calc. | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridine 5'-triphosphate |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridylylated PII signal transduction protein (PII-UMP)50 mM MgCl2; no glutamine | 0.045 | 0.0023 | 20 calc. | pH 7.5 | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridylylated PII signal transduction protein (PII-UMP) |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridylylated PII signal transduction protein (PII-UMP)50 mM MgCl2; 2.5 mM glutamine (saturating activator) | 0.108 | 8.2 × 10⁻⁴ | 130 calc. | pH 7.5 | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridylylated PII signal transduction protein (PII-UMP) |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridylylated PII signal transduction protein (PII-UMP)50 mM MgCl2; 1 mM glutamine | — | 0.0012 | — | pH 7.5 | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridylylated PII signal transduction protein (PII-UMP) |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridylylated PII signal transduction protein (PII-UMP)1 mM MnCl2; no MgCl2; 6 mM glutamine (saturating activator) | 1.02 | 3.2 × 10⁻⁴ | 3.2 × 10³ calc. | pH 7.5 | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridylylated PII signal transduction protein (PII-UMP) |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridylylated PII signal transduction protein (PII-UMP)1 mM MnCl2; no MgCl2; no glutamine | 0.155 | 6.3 × 10⁻⁴ | 250 calc. | pH 7.5 | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridylylated PII signal transduction protein (PII-UMP) |
| uridylyltransferase/uridylyl-removing enzyme (UTase/UR) | Escherichia coli | uridylylated PII signal transduction protein (PII-UMP) | 0.033 | — | — | pH 7.5, 30 °C | Open record: uridylyltransferase/uridylyl-removing enzyme (UTase/UR), uridylylated PII signal transduction protein (PII-UMP) |