EC 2.7.7.6: DNA-directed RNA polymerase

CatLog read 16 records for EC 2.7.7.6 from 1 paper, for 1 organism. Names in these papers: T7 RNA polymerase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 11 records for EC 2.7.7.6 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.6 in Browse.

Papers

Papers with records for EC 2.7.7.6
Paper	Organisms	Records
Determinants of ribose specificity in RNA polymerization: effects of Mn2+ and deoxynucleoside monophosphate incorporation into transcripts	bacteriophage T7	16

Records

Records for EC 2.7.7.6 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
T7 RNA polymerase	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	—	0.387	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.22	0.88	0.25 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	2'-deoxyuridine 5'-triphosphateMgCl2 in	—	1.7	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase, 2'-deoxyuridine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	2'-deoxyuridine 5'-triphosphateMnCl2 replacing the 15 mM MgCl2 of	—	1.2	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase, 2'-deoxyuridine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	guanosine 5'-triphosphate	—	0.0103	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase, guanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	guanosine 5'-triphosphate	0.26	0.25	1.0 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase, guanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	uridine 5'-triphosphateMgCl2 in	—	0.041	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase, uridine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	uridine 5'-triphosphateMnCl2 replacing the 15 mM MgCl2 of	—	0.036	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase, uridine 5'-triphosphate
T7 RNA polymerasevariant S641A	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.32	1.4	0.23 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase variant S641A, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant S641A	bacteriophage T7	guanosine 5'-triphosphate	0.38	0.21	1.8 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase variant S641A, guanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	—	0.025	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase variant Y639F, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.34	0.75	0.45 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase variant Y639F, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	guanosine 5'-triphosphate	—	0.0153	—	pH 8	PMID 9354643	Open record: T7 RNA polymerase variant Y639F, guanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	guanosine 5'-triphosphate	0.28	0.32	0.88 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase variant Y639F, guanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F/S641A	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.1	1.1	0.091 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase variant Y639F/S641A, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F/S641A	bacteriophage T7	guanosine 5'-triphosphate	0.25	0.22	1.1 calc.	pH 8, 37 °C	PMID 9354643	Open record: T7 RNA polymerase variant Y639F/S641A, guanosine 5'-triphosphate