Values CatLog read from this paper

Determinants of ribose specificity in RNA polymerization: effects of Mn2+ and deoxynucleoside monophosphate incorporation into transcripts

PMID 9354643doi:10.1021/bi971609oOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (24 values) from this paper: T7 RNA polymerase from bacteriophage T7.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.7.6

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
T7 RNA polymerase	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	—	0.387	—	pH 8	Open record: T7 RNA polymerase, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.22	0.88	0.25 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	2'-deoxyuridine 5'-triphosphateMgCl2 in	—	1.7	—	pH 8	Open record: T7 RNA polymerase, 2'-deoxyuridine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	2'-deoxyuridine 5'-triphosphateMnCl2 replacing the 15 mM MgCl2 of	—	1.2	—	pH 8	Open record: T7 RNA polymerase, 2'-deoxyuridine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	guanosine 5'-triphosphate	—	0.0103	—	pH 8	Open record: T7 RNA polymerase, guanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	guanosine 5'-triphosphate	0.26	0.25	1.0 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase, guanosine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	uridine 5'-triphosphateMgCl2 in	—	0.041	—	pH 8	Open record: T7 RNA polymerase, uridine 5'-triphosphate
T7 RNA polymerase	bacteriophage T7	uridine 5'-triphosphateMnCl2 replacing the 15 mM MgCl2 of	—	0.036	—	pH 8	Open record: T7 RNA polymerase, uridine 5'-triphosphate
T7 RNA polymerasevariant S641A	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.32	1.4	0.23 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase variant S641A, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant S641A	bacteriophage T7	guanosine 5'-triphosphate	0.38	0.21	1.8 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase variant S641A, guanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	—	0.025	—	pH 8	Open record: T7 RNA polymerase variant Y639F, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.34	0.75	0.45 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase variant Y639F, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	guanosine 5'-triphosphate	—	0.0153	—	pH 8	Open record: T7 RNA polymerase variant Y639F, guanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F	bacteriophage T7	guanosine 5'-triphosphate	0.28	0.32	0.88 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase variant Y639F, guanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F/S641A	bacteriophage T7	2'-deoxyguanosine 5'-triphosphate	0.1	1.1	0.091 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase variant Y639F/S641A, 2'-deoxyguanosine 5'-triphosphate
T7 RNA polymerasevariant Y639F/S641A	bacteriophage T7	guanosine 5'-triphosphate	0.25	0.22	1.1 calc.	pH 8, 37 °C	Open record: T7 RNA polymerase variant Y639F/S641A, guanosine 5'-triphosphate