EC 2.7.7.65: diguanylate cyclase

TransferasesOpen in Browse

CatLog read 14 records for EC 2.7.7.65 from 1 paper, for 1 organism. Names in these papers: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 24 records for EC 2.7.7.65 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.65 in Browse.

Papers

Papers with records for EC 2.7.7.65
Paper	Organisms	Records
Kinetic analysis of a globin-coupled diguanylate cyclase, YddV: Effects of heme iron redox state, axial ligands, and heme distal mutations on catalysis	Escherichia coli	14

Records

Records for EC 2.7.7.65 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphateheme Fe(III); 5-coordinate high-spin; as isolated; Table 1	0.02	0.084	0.23	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphateheme Fe(III)-CN- complex (protein mixed with 0.1 M KCN)	0.0103	0.041	0.25	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphate50 mM Tris-HCl pH 8.0; 5 mM MgCl2; 0-1000 µM GTP; Table 1	0.02	0.131	0.15	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphate50 mM Tris-HCl pH 8.0; 5 mM MgCl2; 0-1000 µM GTP; Table 1	0.0158	0.087	0.18	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphate50 mM Tris-HCl pH 8.0; 5 mM MgCl2; 0-1000 µM GTP; Table 1	0.005	0.039	0.13	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphateheme Fe(II) complex (dithionite-reduced, desalted); Table 1	0.005	0.038	0.13	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphate5 mM MnCl2 (replacing MgCl2); heme Fe(III) form; HPLC	0.017	0.015	1.12	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase	Escherichia coli	guanosine 5'-triphosphate5 mM MgCl2; heme Fe(III) form; 5-1000 µM GTP; HPLC	0.02	0.076	0.27	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65G	Escherichia coli	guanosine 5'-triphosphate	0.0025	0.022	0.12	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65G, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65M	Escherichia coli	guanosine 5'-triphosphate	0.0142	0.042	0.33	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65M, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65Q	Escherichia coli	guanosine 5'-triphosphate	0.0043	0.101	0.05	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65Q, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65T	Escherichia coli	guanosine 5'-triphosphate	0.0078	0.063	0.12	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65T, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant Y43A	Escherichia coli	guanosine 5'-triphosphate	0.009	0.054	0.17	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant Y43A, guanosine 5'-triphosphate
YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant Y43F	Escherichia coli	guanosine 5'-triphosphate	0.0115	0.029	0.4	pH 8, 24 °C	PMID 31520879	Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant Y43F, guanosine 5'-triphosphate