Values CatLog read from this paper
Kinetic analysis of a globin-coupled diguanylate cyclase, YddV: Effects of heme iron redox state, axial ligands, and heme distal mutations on catalysis
PMID 31520879doi:10.1016/j.jinorgbio.2019.110833Open in Browse
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CatLog read 14 records (42 values) from this paper: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphateheme Fe(III); 5-coordinate high-spin; as isolated; Table 1 | 0.02 | 0.084 | 0.23 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphateheme Fe(III)-CN- complex (protein mixed with 0.1 M KCN) | 0.0103 | 0.041 | 0.25 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphate50 mM Tris-HCl pH 8.0; 5 mM MgCl2; 0-1000 µM GTP; Table 1 | 0.02 | 0.131 | 0.15 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphate50 mM Tris-HCl pH 8.0; 5 mM MgCl2; 0-1000 µM GTP; Table 1 | 0.0158 | 0.087 | 0.18 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphate50 mM Tris-HCl pH 8.0; 5 mM MgCl2; 0-1000 µM GTP; Table 1 | 0.005 | 0.039 | 0.13 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphateheme Fe(II) complex (dithionite-reduced, desalted); Table 1 | 0.005 | 0.038 | 0.13 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphate5 mM MnCl2 (replacing MgCl2); heme Fe(III) form; HPLC | 0.017 | 0.015 | 1.12 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase | Escherichia coli | guanosine 5'-triphosphate5 mM MgCl2; heme Fe(III) form; 5-1000 µM GTP; HPLC | 0.02 | 0.076 | 0.27 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65G | Escherichia coli | guanosine 5'-triphosphate | 0.0025 | 0.022 | 0.12 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65G, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65M | Escherichia coli | guanosine 5'-triphosphate | 0.0142 | 0.042 | 0.33 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65M, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65Q | Escherichia coli | guanosine 5'-triphosphate | 0.0043 | 0.101 | 0.05 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65Q, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant L65T | Escherichia coli | guanosine 5'-triphosphate | 0.0078 | 0.063 | 0.12 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant L65T, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant Y43A | Escherichia coli | guanosine 5'-triphosphate | 0.009 | 0.054 | 0.17 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant Y43A, guanosine 5'-triphosphate |
| YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclasevariant Y43F | Escherichia coli | guanosine 5'-triphosphate | 0.0115 | 0.029 | 0.4 | pH 8, 24 °C | Open record: YddV (EcDosC), globin-coupled heme-based oxygen sensor diguanylate cyclase variant Y43F, guanosine 5'-triphosphate |