EC 2.7.7.69: GDP-L-galactose/GDP-D-glucose: hexose 1-phosphate guanylyltransferase
TransferasesOpen in Browse
CatLog read 27 records for EC 2.7.7.69 from 3 papers, for 1 organism. Names in these papers: GDP-L-galactose phosphorylase VTC5, VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), GDP-L-galactose phosphorylase VTC2, VTC2 GDP-L-galactose phosphorylase and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 1 record for EC 2.7.7.69 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.69 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction | Arabidopsis thaliana | 14 |
| Arabidopsis VTC2 encodes a GDP-L-galactose phosphorylase, the last unknown enzyme in the Smirnoff-Wheeler pathway to ascorbic acid in plants | Arabidopsis thaliana | 7 |
| Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability | Arabidopsis thaliana | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| GDP-L-galactose phosphorylase VTC2 | Arabidopsis thaliana | alpha-D-glucose 1-phosphate | — | 26 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC2, alpha-D-glucose 1-phosphate |
| GDP-L-galactose phosphorylase VTC2 | Arabidopsis thaliana | GDP-beta-L-galactose | 27 | 0.0079 | 3.4 × 10³ | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC2, GDP-beta-L-galactose |
| GDP-L-galactose phosphorylase VTC2 | Arabidopsis thaliana | phosphatephosphorylase reaction varying Pi; 25 µM GDP-L-Gal | — | 2.4 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC2, phosphate |
| GDP-L-galactose phosphorylase VTC2 | Arabidopsis thaliana | phosphatephosphorylase reaction varying Pi; 50 µM GDP-D-Glc | — | 0.76 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC2, phosphate |
| GDP-L-galactose phosphorylase VTC2 | Arabidopsis thaliana | phosphateFig. 4 saturation curve; 25 µM GDP-L-Gal; 10 min reactions | — | 1.8 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC2, phosphate |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | alpha-D-glucose 1-phosphatereverse reaction; 5 mM GDP; no phosphate or MgCl2 | 5.8 | 10 | 0.58 | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, alpha-D-glucose 1-phosphate |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | alpha-D-glucose 1-phosphateFig. 5 saturation curve; 2 mM MgCl2; 25 µM GDP-L-Gal | — | 6.8 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, alpha-D-glucose 1-phosphate |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | beta-L-galactose 1-phosphate | 3.8 | 16 | 0.25 | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, beta-L-galactose 1-phosphate |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | GDP-alpha-D-glucose | 9.9 | 0.012 | 860 | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, GDP-alpha-D-glucose |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | GDP-alpha-D-mannose | 0.024 | 1.3 | 0.019 | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, GDP-alpha-D-mannose |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | GDP-beta-L-galactose | 13 | 0.0083 | 1.6 × 10³ | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, GDP-beta-L-galactose |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | phosphatephosphorylase reaction varying Pi; 25 µM GDP-L-Gal | — | 1 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, phosphate |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | phosphatephosphorylase reaction varying Pi; 50 µM GDP-D-Glc | — | 0.22 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, phosphate |
| GDP-L-galactose phosphorylase VTC5 | Arabidopsis thaliana | phosphateFig. 5 saturation curve; 25 µM GDP-L-Gal; 10 min reactions | — | 0.96 | — | pH 7.5, 26 °C | PMID 18463094 | Open record: GDP-L-galactose phosphorylase VTC5, phosphate |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | alpha-D-galactose 1-phosphate | 0.023 | 67 | 3.4 × 10⁻⁴ | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), alpha-D-galactose 1-phosphate |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | alpha-D-mannose 1-phosphate | 0.0013 | 54 | 2.5 × 10⁻⁵ | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), alpha-D-mannose 1-phosphate |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | beta-L-galactose 1-phosphate | 6 | 45 | 0.133 | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), beta-L-galactose 1-phosphate |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | D-glucose 1-phosphate | 12 | 29 | 0.412 | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), D-glucose 1-phosphate |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | GDP-alpha-D-glucose | 23 | 0.0044 | 5.7 × 10³ | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), GDP-alpha-D-glucose |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | GDP-alpha-D-mannose | 0.093 | 0.52 | 0.19 | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), GDP-alpha-D-mannose |
| VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase) | Arabidopsis thaliana | GDP-L-galactose | 64 | 0.01 | 6.3 × 10³ | pH 7.5, 26 °C | PMID 17462988 | Open record: VTC2 (GDP-L-galactose/GDP-D-glucose phosphorylase), GDP-L-galactose |
| VTC2 GDP-L-galactose phosphorylase | Arabidopsis thaliana | GDP-beta-L-galactose | 2.04 | 0.25 | 8.16 | pH 6.9, 20 °C | PMID 17877701 | Open record: VTC2 GDP-L-galactose phosphorylase, GDP-beta-L-galactose |
| VTC2 GDP-L-galactose phosphorylase | Arabidopsis thaliana | phosphate | — | 0.251 | 8.11 | pH 6.9, 20 °C | PMID 17877701 | Open record: VTC2 GDP-L-galactose phosphorylase, phosphate |
| VTC2 GDP-L-galactose phosphorylasevariant S290F | Arabidopsis thaliana | GDP-beta-L-galactose | 0.41 | 0.123 | 3.32 | pH 6.9, 20 °C | PMID 17877701 | Open record: VTC2 GDP-L-galactose phosphorylase variant S290F, GDP-beta-L-galactose |
| VTC2 GDP-L-galactose phosphorylasevariant S290F | Arabidopsis thaliana | phosphate | — | 2.5 | 0.16 | pH 6.9, 20 °C | PMID 17877701 | Open record: VTC2 GDP-L-galactose phosphorylase variant S290F, phosphate |
| VTC5 (At5g55120) GDP-L-galactose phosphorylase | Arabidopsis thaliana | GDP-beta-L-galactose | 2.68 | 0.667 | 4.02 | pH 6.9, 20 °C | PMID 17877701 | Open record: VTC5 (At5g55120) GDP-L-galactose phosphorylase, GDP-beta-L-galactose |
| VTC5 (At5g55120) GDP-L-galactose phosphorylase | Arabidopsis thaliana | phosphate | — | 0.13 | 20.55 | pH 6.9, 20 °C | PMID 17877701 | Open record: VTC5 (At5g55120) GDP-L-galactose phosphorylase, phosphate |