CatLogenzyme kinetics catalog

Values CatLog read from this paper

A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction

PMID 18463094doi:10.1074/jbc.m802594200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (30 values) from this paper: GDP-L-galactose phosphorylase VTC5, GDP-L-galactose phosphorylase VTC2, GDP-L-galactose phosphorylase (endogenous activity) and 1 more from Arabidopsis thaliana, Brassica rapa var. komatsuna.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.69

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
GDP-L-galactose phosphorylase (endogenous activity)Brassica rapa var. komatsunaalpha-D-glucose 1-phosphate—13—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase (endogenous activity)Brassica rapa var. komatsunaphosphate—1.4—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase (endogenous activity, VTC2 + VTC5)Arabidopsis thalianaalpha-D-glucose 1-phosphate—18—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase (endogenous activity, VTC2 + VTC5)Arabidopsis thalianaphosphate—1.1—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC2Arabidopsis thalianaalpha-D-glucose 1-phosphate—26—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC2Arabidopsis thalianaGDP-beta-L-galactose270.00793.4 × 10³pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC2Arabidopsis thalianaphosphatephosphorylase reaction varying Pi; 25 µM GDP-L-Gal—2.4—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC2Arabidopsis thalianaphosphatephosphorylase reaction varying Pi; 50 µM GDP-D-Glc—0.76—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC2Arabidopsis thalianaphosphateFig. 4 saturation curve; 25 µM GDP-L-Gal; 10 min reactions—1.8—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaalpha-D-glucose 1-phosphatereverse reaction; 5 mM GDP; no phosphate or MgCl25.8100.58pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaalpha-D-glucose 1-phosphateFig. 5 saturation curve; 2 mM MgCl2; 25 µM GDP-L-Gal—6.8—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianabeta-L-galactose 1-phosphate3.8160.25pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaGDP-alpha-D-glucose9.90.012860pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaGDP-alpha-D-mannose0.0241.30.019pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaGDP-beta-L-galactose130.00831.6 × 10³pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaphosphatephosphorylase reaction varying Pi; 25 µM GDP-L-Gal—1—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaphosphatephosphorylase reaction varying Pi; 50 µM GDP-D-Glc—0.22—pH 7.5, 26 °COpen
GDP-L-galactose phosphorylase VTC5Arabidopsis thalianaphosphateFig. 5 saturation curve; 25 µM GDP-L-Gal; 10 min reactions—0.96—pH 7.5, 26 °COpen