CatLogenzyme kinetics catalog

EC 2.7.7.72: CCA tRNA nucleotidyltransferase

TransferasesOpen in Browse

CatLog read 20 records for EC 2.7.7.72 from 2 papers, for 4 organisms. Names in these papers: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCA, CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCA.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 50 records for EC 2.7.7.72 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.7.72 in Browse.

Papers

PaperOrganismsRecords
Adaptation of the Romanomermis culicivorax CCA-Adding Enzyme to Miniaturized Armless tRNA SubstratesHomo sapiens, Romanomermis culicivorax12
Poly(C) synthesis by class I and class II CCA-adding enzymesEscherichia coli, Sulfolobus shibatae8

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Escherichia coliadenosine 5'-triphosphate0.2650.1441.837 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Escherichia colicytidine 5'-triphosphate0.01220.3320.03737 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Escherichia colireconstituted E. coli tRNAAla Ala-75C (57-mer 1-57 annealed with 18-mer Ala-18C ending with CC)CTP addition (C76) to the CC terminus; no ATP0.00130.1450.009237 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Escherichia colireconstituted E. coli tRNAAla Ala-75C (57-mer 1-57 annealed with 18-mer Ala-18C ending with CC)ATP addition (A76) to the CC terminus; cosubstrate ATP0.0180.0540.3337 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Escherichia coliVal-34C minihelix (acceptor-TPsiC domain of E. coli tRNAVal, 34-mer ending with C75)CTP addition (C76) to the CC terminus; no ATP0.00120.3660.003337 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Escherichia coliVal-34C minihelix (acceptor-TPsiC domain of E. coli tRNAVal, 34-mer ending with C75)ATP addition (A76) to the CC terminus; cosubstrate ATP0.0220.2630.08337 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Sulfolobus shibataeadenosine 5'-triphosphate0.0350.50.0770 °CPMID 11926813Open
CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase)Sulfolobus shibataecytidine 5'-triphosphate0.00270.5150.005270 °CPMID 11926813Open
CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCAHomo sapiensRomanomermis culicivorax armless mitochondrial tRNAArg in vitro transcript lacking the CCA-endcosubstrate ATP (with CTP; 1 mM NTPs)0.0030.004770.63 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCAHomo sapiensRomanomermis culicivorax armless mitochondrial tRNAArg in vitro transcript lacking the CCA-endC*C* reaction: CC-addition measured with alpha-32P-CTP0.0410.001332 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCAHomo sapiensRomanomermis culicivorax armless mitochondrial tRNAIle in vitro transcript lacking the CCA-endcosubstrate ATP (with CTP; 1 mM NTPs)0.0060.0023.0 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCAHomo sapiensRomanomermis culicivorax armless mitochondrial tRNAIle in vitro transcript lacking the CCA-endC*C* reaction: CC-addition measured with alpha-32P-CTP0.1650.0055829.6 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCAHomo sapiensSaccharomyces cerevisiae cytosolic tRNAPhe in vitro transcript lacking the CCA-endcosubstrate ATP (with CTP; 1 mM NTPs)0.0910.0042821 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), HsaCCAHomo sapiensSaccharomyces cerevisiae cytosolic tRNAPhe in vitro transcript lacking the CCA-endC*C* reaction: CC-addition measured with alpha-32P-CTP0.2140.0041251.9 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCARomanomermis culicivoraxRomanomermis culicivorax armless mitochondrial tRNAArg in vitro transcript lacking the CCA-endcosubstrate ATP (with CTP; 1 mM NTPs)0.0120.00139.2 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCARomanomermis culicivoraxRomanomermis culicivorax armless mitochondrial tRNAArg in vitro transcript lacking the CCA-endC*C* reaction: CC-addition measured with alpha-32P-CTP0.0810.0016948 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCARomanomermis culicivoraxRomanomermis culicivorax armless mitochondrial tRNAIle in vitro transcript lacking the CCA-endcosubstrate ATP (with CTP; 1 mM NTPs)0.0520.007846.6 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCARomanomermis culicivoraxRomanomermis culicivorax armless mitochondrial tRNAIle in vitro transcript lacking the CCA-endC*C* reaction: CC-addition measured with alpha-32P-CTP0.2240.0054341.3 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCARomanomermis culicivoraxSaccharomyces cerevisiae cytosolic tRNAPhe in vitro transcript lacking the CCA-endcosubstrate ATP (with CTP; 1 mM NTPs)0.0410.004668.8 calc.20 °CPMID 33260740Open
CCA-adding enzyme (tRNA nucleotidyltransferase), RcuCCARomanomermis culicivoraxSaccharomyces cerevisiae cytosolic tRNAPhe in vitro transcript lacking the CCA-endC*C* reaction: CC-addition measured with alpha-32P-CTP0.1660.0030155.1 calc.20 °CPMID 33260740Open