Values CatLog read from this paper
Poly(C) synthesis by class I and class II CCA-adding enzymes
PMID 11926813doi:10.1021/bi0120953Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) from Escherichia coli, Methanococcus jannaschii, Sulfolobus shibatae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Escherichia coli | adenosine 5'-triphosphate | 0.265 | 0.144 | 1.8 | 37 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), adenosine 5'-triphosphate |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Escherichia coli | cytidine 5'-triphosphate | 0.0122 | 0.332 | 0.037 | 37 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), cytidine 5'-triphosphate |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Escherichia coli | reconstituted E. coli tRNAAla Ala-75C (57-mer 1-57 annealed with 18-mer Ala-18C ending with CC)CTP addition (C76) to the CC terminus; no ATP | 0.0013 | 0.145 | 0.0092 | 37 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), reconstituted E. coli tRNAAla Ala-75C (57-mer 1-57 annealed with 18-mer Ala-18C ending with CC) |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Escherichia coli | reconstituted E. coli tRNAAla Ala-75C (57-mer 1-57 annealed with 18-mer Ala-18C ending with CC)ATP addition (A76) to the CC terminus; cosubstrate ATP | 0.018 | 0.054 | 0.33 | 37 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), reconstituted E. coli tRNAAla Ala-75C (57-mer 1-57 annealed with 18-mer Ala-18C ending with CC) |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Escherichia coli | Val-34C minihelix (acceptor-TPsiC domain of E. coli tRNAVal, 34-mer ending with C75)CTP addition (C76) to the CC terminus; no ATP | 0.0012 | 0.366 | 0.0033 | 37 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), Val-34C minihelix (acceptor-TPsiC domain of E. coli tRNAVal, 34-mer ending with C75) |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Escherichia coli | Val-34C minihelix (acceptor-TPsiC domain of E. coli tRNAVal, 34-mer ending with C75)ATP addition (A76) to the CC terminus; cosubstrate ATP | 0.022 | 0.263 | 0.083 | 37 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), Val-34C minihelix (acceptor-TPsiC domain of E. coli tRNAVal, 34-mer ending with C75) |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Methanococcus jannaschii | adenosine 5'-triphosphate | 0.192 | 0.287 | 0.67 | 70 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), adenosine 5'-triphosphate |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Methanococcus jannaschii | cytidine 5'-triphosphate | 0.02 | 0.43 | 0.047 | 70 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), cytidine 5'-triphosphate |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Sulfolobus shibatae | adenosine 5'-triphosphate | 0.035 | 0.5 | 0.07 | 70 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), adenosine 5'-triphosphate |
| CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase) | Sulfolobus shibatae | cytidine 5'-triphosphate | 0.0027 | 0.515 | 0.0052 | 70 °C | Open record: CCA-adding enzyme (ATP(CTP):tRNA nucleotidyl transferase), cytidine 5'-triphosphate |